Studies on induction of chlorophyll mutations in soybean, Glycine max (L.) Merrill

Abstract

The phenotypic response of two soybean cultivars to a chemical mutagen (ethyl methane sulphonate, EMS), physical mutagen (gamma rays) and their combinations were studied in M₁ and M₂ generations and the frequency and spectrum of chlorophyll mutations were worked out. Combined treatment was found to be more effective in inducing chlorophyll mutations compared to individual treatments of gamma rays and EMS in both the cultivars. As far as the spectrum of chlorophyll mutations is concerned, a wider spectrum in both the cultivars was observed in 45 kR + 0.2% EMS combined treatment.

Keywords

chlorophyll mutations / gamma rays / ethyl methane sulphonate / soybean

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Acknowledgements

This research was financially supported by the Kisan P.G. College, Simbhaoli, Ghaziabad, U.P, India and Ch. Charan Singh, University, Meerut, U.P, India.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg