Early stage SSH library construction of wheat near isogenic line TcLr19 under the stress of Puccinia recondita f. sp. tritici

Abstract

cDNA library of wheat near isogenic line TcLr19 was constructed with suppression subtractive hybridization (SSH) 16 h after inoculation with race 366 of Puccinia recondita f. sp. tritici. This SSH library included 1337 positive clones and the insert sizes ranged from 200 bp to 600 bp, 237 clones were selected according to the result of reverse northern blotting, and then 35 ESTs were sequenced. EST similarity analysis was finished by comparing sequences with BLAST software in the non-redundant database of GenBank. The results showed that they were related to many biological processes including signal transduction, transcription regulation and hypersensitive response.

Keywords

TcLr19 / leaf rust / suppression subtractive hybridization (SSH) / early stage / ESTs

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 30671244), the Key Basic Research Project of Applied Basic Research Program of Hebei Province, China (No. 08965505D) and the Natural Science Foundation of Hebei Province, China (Nos. 303180, C2005000220 and C2007000515).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg