Genetic diversity of Robinia pseudoacacia populations in China detected by AFLP markers

Abstract

The genetic diversity of ten Robinia pseudoacacia L. populations collected from China was analyzed by amplified fragment length polymorphism (AFLP) technique using ten primer combinations. A total of 752 amplified bands were obtained, among which 352 (46.8%) were polymorphic. At species level, the percentage of polymorphic loci (P) was 49.87%, the Shannon’s information index (I) was 0.2160, and the mean Nei’s gene diversity index (H) was 0.1403. At population level, P=25.47%, I=0.1381, and H=0.0927. The genetic diversity within populations was higher than that among populations. The coefficient of gene differentiation among populations within species (Gst) was 0.390, which indicated that gene differentiation was mainly within the population, and between populations, it accounted for 33.90% of the total variation. Gene flow (Nm) between the populations was 0.975, suggesting that the gene exchange between populations was small. The UPGMA cluster analysis showed that the ten populations were divided into three major groups, and most individuals from the same population were clustered together. There was no significant correlation between the genetic diversity parameters (D, I_N, P, Ne, H, and I) and geographic and climatic factors (longitude, latitude, annual mean temperature, and annual mean precipitation). The results provide useful information about the level of genetic diversity, and it has a wide application prospect in Robinia pseudoacacia L. utilization and breeding in China.

Keywords

amplified fragment length polymorphism (AFLP) / genetic diversity / genetic structure / population / Robinia pseudocacia L.

References

[1] Brown A H D, Moran G F (1981). Isozymes and the genetic resources of forest trees. In: Conkte M T, ed. Proceedings of a Symposium on Isozymes of North American Forest Trees and Forest Insects. NC: USA For Serv Gen Tech Rep, PSW-48, 1-10

[2] Chang C S, Bongarten B, Hamrick J (1998). Genetic structure of natural populations of black locust (Robinia pseudoacacia L.) at Coweeta, North Carolina. Journal of Plant Research, 111: 17-24

[3] Cheng L L, Su S C, Qin L, Yin W L (2005). Extraction of DNA and establishment of AFLP techniques in leaves of Castanea mollissima Blume. Journal of Beijing Agricultural College, 20(2): 5-10 (in Chinese)

[4] Doyle J J, Doyle J L (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin, 19: 11-15

[5] Gu J T, Yang M S, Li Y M (2006). Allozyme genetic investigation on Robinia pseudoacacia. Scientia Silvae Sinicae, 42(11): 138-145 (in Chinese)

[6] Hamrick J L, Godt M J W, Sherman-Broyles S L (1992). Factors influencing levels of genetic diversity in woody plant species. New Forests, 6: 95-124

[7] Hill M, Witsenboer H, Zabeau M, Vos P, Kesseli R, Michelmore R (1996). PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp. Theoretical and Applied Genetics, 93(8): 1202-1210

[8] Lang P, Huang H W (1999). Genetic diversity and geographic variation in natural populations of the Endemic castanea species in China. Acta Botanica Sinica, 41(6): 651-657 (in Chinese)

[9] Li W Y, Gu W C, Zhou S L (2003). AFLP analysis on genetic diversity of Quercus mongolica populations. Scientia Silvae Sinicae, 39(5): 29-36 (in Chinese)

[10] Li Z B, Lin P (2001). The study on genetic diversity of Aegiceras corniculatum populations in different latitudes. Journal of Jimei University, 6(1): 39-45 (in Chinese)

[11] Liesebach H, Yang M S, Schneck V (2004). Genetic diversity and differentiation in a black locust (Robinia pseudoacacia L.) progeny test. Forest Genetics, 11(2): 151-161

[12] Loveless M D, Hamrick J L H (1984). Ecological determinants of genetic structure in plant populations. Annual Review Ecology System, 15: 65-95

[13] Major A, Malvolti M E, Cannata F (1998). Comparison of isozyme and RAPD variability of black locust (Robinia pseudoacacia) clones selected for silvicultural objectives. Journal of Genetics & Breeding, 52: 49-62

[14] Nei M (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy Sciences, USA, 70(12): 3321-3323

[15] Peng H, Chen X R, Yu Z D (2003). Know-how on silviculture of black locust plantation. Journal of Soil and Water Conservation, 17(5): 11-15 (in Chinese)

[16] Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2(3): 225-238

[17] Redei K (1999). Black locust (Robinia pseudoacacia L.) improvement and management in Hungary. Forestry Studies in China, 11(2): 42-46

[18] Shim S I, Jorgensen R B (2000). Genetic structure in cultivated and wild carrots (Daucus carota L.) revealed by AFLP analysis. Theoretical and Applied Genetics, 101: 227-233

[19] Song H Z, Zhang Q W, Zhou C J (2007). Analysis of genetic diversity of some species in Populus by AFLP marker. Scientia Silvae Sinicae, 43(12): 64-69 (in Chinese)

[20] Surles S E, Hamrick J L, Bongarten B C (1989). Allozyme in black locust (Robinia pseudoacacia). Canadian Journal of Forest Research, 19(44): 471-479

[21] Vos P, Hogers R, Bleeker M, Reijans M, Lee T V D, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23(21): 4407-4414

[22] Wang L, Xing S Y, Yang K Q, Wang Z H, Gu Y Y, Shu H R (2006). Genetic relationships of ornamental cultivars of Ginkgo biloba analyzed by AFLP techniques. Acta Genetica Sinica, 33(11): 1020-1026

[23] Wang X R, Zhao Y, Chen X Y (2007). AFLP analysis of genetic diversity among Lespedeza bicolor populations. Bolletin of Botanical Research, 27(6): 736-740 (in Chinese)

[24] Yang M S, Heike H, Volker S (2004). Genetic diversity and population structure of Robinia pseudoacacia provenances from middle Europe. Acta Ecologica Sinica, 24(12): 2700-2706 (in Chinese)

[25] Yeh F C, Yang R C, Boyle T B J, Ye Z H, Mao J X (1997). Popgene, the user-friendly shareware for population genetic analysis. Edmonton: University of Alberta, Molecular Biology and Biotechnology Centre

[26] Yuan Z H, Chen X S, He T M, Feng J R, Feng T, Zhang C Y (2007a). Population genetic structure in apricot (Prunus armeniaca L.) cultivars revealed by fluorescent-AFLP markers in southern Xinjiang, China. Journal of Genetics and Genomics, 34(11): 1037-1047

[27] Yuan Z H, Yin Y L, Qu J L, Zhu L Q, Li Y (2007b). Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. Journal of Genetics and Genomics, 34(12): 1061-1071

[28] Zhang J L, Wang Z F, Li M G, Wang B S (2000). Molecular markers and their application in plant population research. Chinese Journal of Applied Ecology, 11(4): 631-636 (in Chinese)

[29] Zhang L, Huang B B, Kai G Y, Guo M L (2006). Analysis of intraspecific variation of Chinese Carthamus tinctorius L. using AFLP markers. Acta Pharmaceutica Sinica, 41(1): 91-96 (in Chinese)

[30] Zhang P, Liu J S, Jin C D, Zhang Y Z (2002). Cultivation and using status of Robinia pesudoacacia. Journal of Agricultural Science Yanbian University, 24(2): 223-227 (in Chinese)

Acknowledgements

We sincerely thank Prof. Haiyong Liang at the Forestry College of Agricultural University of Hebei, China and Dr. Juntao Gu, at the College of Life Science of Agricultural University of Hebei, China for their contributions to our work. This research was financially supported by the State Forestry Administration “948” Project of China (No. 2005-4-41).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg