Detection of Eperythrozoon wenyoni by PCR assay

Abstract

The objective of this research was to develop a detection method for Eperythrozoon wenyoni infection using polymerase chain reaction (PCR) assay technique. A pair of primers was designed and synthesized according to the conservative sequence 16S rRNA. The PCR assay was performed with the primers. A 985-bp fragment was amplified by using PCR. The amplified fragments with the expected size were identified by EcoR I restriction digestion. The crossing-reaction, specific-reaction and duplicate-reaction indicated that the PCR method is a specific, sensitive, fast and effective method for diagnosing E. wenyoni infection at group level.

Keywords

cattle / Eperythrozoon wenyoni / PCR

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Acknowledgements

This research was supported by the National Science and Technology Tackle Key Problem Project Foundation of China (No. 2006BAD04A10-4), Hebei Significant Technology Special Planned Project, China (No. 07227146Z-4), and the Science and Technology Research Project Foundation from Hebei Bureau of Animal Science, China (No. 2004-14).

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