Directional transduction of male sterile gene of NIAN type in wheat

Abstract

A new method for producing a NIAN type wheat maintenance line with the male sterile gene rfv₁ was described. That is the variety Xinong Fp1, a 1BL/1RS translocation line, as the acceptor and Triticum macha var. subletschchumicum, a non-1BL/1RS translocation line, as the donor, a directional substitution backcross was made and confirmed by chromosome of root tip preparations and SDS-PAGE analysis. The male sterile gene rfv₁ of Triticum macha var. subletschchumicum was transferred to the genome of Xinong Fp1. A new NIAN type wheat maintenance line with the male sterile gene rfv₁ was bred. The method described was successful in breeding a new male sterile type for hybrid wheat production.

Keywords

wheat / male sterile lines of NIAN type / 1BL/1RS translocation chromosome / gene rfv₁ / directional transduction