Effect of CaCl treatment on the changing of drought related physiological and biochemical indexes of

Abstract

Some experiments revealed that the Ca²⁺ has a relationship with drought resistance. In this paper, some physiological and biochemical indices were studied in order to analyze the effect of Ca²⁺ treatment on the drought resistance of Brassica napus. The physiological and biochemical experiments revealed that the proline content and the soluble sugar contents in the Ca²⁺-treated B. napus were much higher than those of water-treated B. napus. However, the content of malonaldehyde in the Ca²⁺ treated B. napus was lower than that of control. The transpiration and stomatal conductance analysis show that the transpiration and stomatal conductance in the Ca²⁺-treated materials were lower than those of water-treated materials. Further research revealed that the stomata hatch of Ca²⁺-treated materials was markedly less than that of water-treated materials, which indicated that the closure of the stomata was the main reason for the decrease of transpiration and stomatal conductance.

References

1. Bartels D, Sunkar R (2005). Droughtand salt tolerance in plants. CriticalReviews in Plant Sciences, 24: 23–58. doi:10.1080/07352680590910410
2. Bonilla I, El-Hamdaoui A, Bolaños L (2004). Boron and calcium increase Pisum sativum seed germination and seedlingdevelopment under salt stress. Plant andSoil, 267: 97–107. doi:10.1007/s11104-005-4689-7
3. Braam J, Davis R W (1990). Rain-,wind-, and touch-induced expression of calmodulin and calmodulin-relatedgenes in Arabidopsis. Cell, 60: 357–364. doi:10.1016/0092-8674(90)90587-5
4. Cai H, Biswas D K, Shang A Q, Zhao L J, Li W D (2007). Photosynthetic responseto water stress and changes in metabolites in Jasminum sambac. Photosynthetica, 45(4): 503–509. doi:10.1007/s11099-007-0087-0
5. Cechin I, Rossi S C, Oliveira V C, Fumis T F (2006). Photosynthetic responses and proline content of mature and youngleaves of sunflower plants under water deficit. Photosynthetica, 44: 143–146. doi:10.1007/s11099-005-0171-2
6. Cheng H L, Tang L S, Zhang Y G, Yan J Y, Zhang H (1997). The effect of CaCl₂ on drought resistance of cotton seedlings. China Cotton, 24(12): 17–18 (in Chinese)
7. Fu T D (2000). The Utilization of Hybrid Rapeseed Breeding. Wuhan: HubeiScientific Technology Press (in Chinese)
8. Hong Z, Lakkineni K, Zhang Z, Verma D P S (2000). Removal of feedback inhibition of 1-pyrroline-5-carboxylatesynthetase results in increased proline accumulation and protectionof plants from osmotic stress. Plant Physiology, 122: 1129–1136. doi:10.1104/pp.122.4.1129
9. Hu H, Dai M, Yao J, Xaio B, Li X, Zhang Q, Xiong L (2006). Overexpressing aNAM, ATAF, and CUC (NAC) transcription factor enhances drought resistanceand salt tolerance in rice. Proceedingof the National Academy of Science, 103(35): 12987–12992. doi:10.1073/pnas.0604882103
10. Joel A, Wu Y, Chang H (2002). Transcriptome changes for Arabidopsis in response to salt, osmotic,and cold stress. Plant Physiology, 130: 2129–2141. doi:10.1104/pp.008532
11. Kiegle E, Moore C A, Haseloff J, Tester M A, Knight M R (2000). Celltype-specificcalcium responses to drought, salt and cold in the Arabidopsis root. Plant Journal, 23: 267–278. doi:10.1046/j.1365-313x.2000.00786.x
12. Knight H (2000). Calcium signaling during abiotic stress in plants. Int Rev Cytol, 195: 269–325. doi:10.1016/S0074-7696(08)62707-2
13. Li H S (2000). The Principle and Technology of Plant Physiology andBiochemistry. Beijing: Higher Education Press (in Chinese)
14. Miyashita K, Tanakamaru S, Maitani T, Kimura K (2005). Recovery responses of photosynthesis, transpiration, and stomataconductance in kidney bean following drought stress. Environmental and Experimental Botany, 53: 205–214. doi:10.1016/j.envexpbot.2004.03.015
15. Qiu D Y, Hu L Y (2002). Effectof nitrogen levels on endogenous hormones content of rapeseed (Brassica napus L.). Journal of Huazhong Agricultural University, 21(3): 213–216 (in Chinese)
16. Rontein D, Basset G, Hanson A D (2002). Metabolic engineering of osmoprotectantaccumulation in plants. Metabolic Eng, 4: 49–56. doi:10.1006/mben.2001.0208
17. Souza R P, Machado E C, Silva J A B, Lagôa A M M A, Silveira J A G (2004). Photosyntheticgas exchange, chlorophyll fluorescence and some associated metabolicchanges in cowpea (Vigna unguiculata) during water stress and recovery. Environmentaland Experimental Botany, 51: 45–56. doi:10.1016/S0098-8472(03)00059-5
18. Wei A L, Chang Z J, Xing Y, Li J (2006). Comparison of stomata features of octoploid Tritielytrigia varieties and Triticumaestivum varieties with different water-requirements. Acta Botanica Boreali-Occidentalia Sinica, 26 (8): 1727–1731
19. Xu H, Heath M C (1998). Roleof calcium in signal transduction during the hypersensitive responsecaused by basidiospore-derived infection of the cowpea rust fungus. Plant Cell, 10: 585–597. doi:10.1105/tpc.10.4.585
20. Zhang M Q (2005). The Molecular Physiology and Genetic Improvement. Beijing: Science Press (inChinese)