Effects of EGF or bFGF on the development of porcine parthenogenetic embryos

Abstract

Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were added into the culture medium in different culturing stages. The effects of EGF or bFGF on the development of porcine parthenogenetic embryos were studied in vitro. The results were as follows: The addition of EGF significantly enhanced the cleavage rate of porcine parthenogenetic embryos (P < 0.05). The addition of EGF or bFGF also significantly enhanced the rate of blastocysts formation of 2–4-cell porcine parthenogenetic embryos (P < 0.05). Additionally, the group of bFGF had more numbers of blastocysts and higher rates of blastocysts formation than the groups of EGF and the control. In conclusion, EGF and bFGF were found propitious to the development of porcine parthenogenetic embryos in vitro, and bFGF increased the quality of blastocysts by increasing the total cell number in porcine parthenogenetic embryos.

References

1. Abeydeera L R, Wang W H, Cantley T C, Rieke A, Prather R S, Day B N (1998). Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryocuture can modulate blastocyst development after in vitro fertilization. MolReprod Dev, 51(4): 395–401. doi:10.1002/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y
2. Anupma G, Fuller W B, Laurie A J (1997). Immunolocalization of acidic andbasic fibroblast growth factors in porcine uterine and conceptus tissues. Biol Reprod, 56(6): 1527–1536. doi:10.1095/biolreprod56.6.1527
3. Campbell W J, Miller K A, Anderson T M, Shull J D, Rizzino A (1992). Expression of fibroblastgrowth factor receptors by embryonal carcinoma cells and early mouseembryos. In Vitro Cell Dev Biol, 28(1): 61–66. doi:10.1007/BF02631080
4. Cohn S (1960). Purification of a nerve-growth promoting protein fromthe mouse salivary gland and its neuro-cytotoxic antiserum. Proc Natl Acad Sci USA, 46(3): 302–311. doi:10.1073/pnas.46.3.302
5. Cutting R C, Pritchard J, Clarke H S, Martin K L (2004). Establishing quality control in the new IVF laboratory. Hum Fertil (Camb), 7(2): 119–125
6. Hrabe de Angelis M, Grundker C, Herrmann B G, Kispert A, Kirchner C (1995). Promotionof gastrulation by maternall growth factor in cultured rabbit blastocysts. Cell Tissue Res, 282(1): 147–154. doi:10.1007/BF00319141
7. Kaye P L (1997). Preimpantation growth factor physiology. Rev Reprod, 2: 373–386
8. Lee G S, Kim H S, Hyun S H, Jeon H Y, Nam D H, Jeong Y W, Kim S, Kim J H, Kang S K, Lee B C, Hwang W S (2005). Effect of epidermal growth factor in preimplantationdevelopment of porcine cloned embryos. Mol Reprod Dev, 71(1): 45–51. doi:10.1002/mrd.20098
9. Pan D K (2005). Factors affecting developmental competence of porcinecloned embryos by somatic cell nuclear transfer. Dissertation forthe Doctoral Degree. Beijing: China Agricultural University, 57–59 (in Chinese)
10. Watson A J, Watson P H, Arcellana-Panlilio M, Warnes D, Walker S K, Schultz G A, Armstrong D T, Seamark R F (1994). A growthfactor phenotype map for ovine preimplantation development. Biol Reprod, 50: 725–733. doi:10.1095/biolreprod50.4.725
11. Wei Z, park K W, Day B N, Prather R S (2001). Effect of epidermal growth factor on preimplantationdevelopment and its receptor expression in porcine embryos. Mol Reprod Dev, 60(4): 457–462. doi:10.1002/mrd.1110