Effect of root exudates of different resistant varieties of cucumber on fusarium wilt and preliminary studies on their resistance mechanism

Abstract

This study investigated the effect of root exudates of cucumber varieties, Jinyan 4 (susceptible variety), Jinchun 4 (resistant variety) and Yinnan Black seed squash on fusarium wilt. The results showed that fusarium wilt occurrence of plants treated with the root exudate of Jinyan 4 was earlier. The infection rate was significantly higher 15 days after inoculation, but similar to the control 20 days after inoculation. In contrast, the infection rate of plants treated with the root exudate of Jinchun 4 was significantly lower than that of the control. The plant height and fresh weight of Jinyan 4 treated with its own root exudate were lower than those of the control, and the root vigor decreased but the conductance increased. There was no significant effect of the root exudates from Jinchun 4 and Black seed squash on plant height and fresh mass of Jinyan 4. We found that the root exudate of susceptible cucumber variety stimulated the growth of Fusarium oxysporum pathogen, in contrast, that of resistance variety and Black seed squash suppressed the growth.

References

1. Chen L F Xu J Y 2001 AgriculturalPlant PathologyBeijingChina Agricultural Press328(in Chinese)
2. Han L M Wang S Q Ju H Y Yan F Li G Q Liu J P Yan J C 2000 Identificationand study on allelopathy of soybean root exudatesSoybean Science19(2)119125(in Chinese)
3. Huang B L Zhu H Zhu F Liu X Y 2004a Occurrence of eggplant wilt and factors affecting growth of Verticillium dahliaeActa Phytophylacica Sinica31(2)157160(in Chinese)
4. Huang B L Zhu H Zhu F Liu X Y 2004b Occurrence reason and control of lettuce rot disease in green houseJiangsu Agricultural Science45051(in Chinese)
5. Jia X M Jiang S J Yin K D Fan W Y 1997 The effect of soybean root system secretion upon the pathogenic fungiproduction by root rot under continuous-cropping and one year intermittentcroppingJournal of Heilongjiang AugustFirst Land Reclamation University9(3)1215(in Chinese)
6. Ju H Y Han L M Wang S Q Cong D L 2002 Allelopathic effects of root exudates on pathogenic fungi of rootrot in continuous cropping soybeanChineseJournal of Applied Ecology13(6)723727(in Chinese)
7. Larkin R P Hopkins D L Martin F N 1993 Effect of successive watermelon plantingson Fusarium oxysporum and othermicro-organisms in soils suppressive and conductive to Fusarium Wiltof watermelonPhytopathology8310971104. doi:10.1094/Phyto‐83‐1097
8. Lü S Z Ma D H Huo Z R Shen W Y Li S J Chen Z W 1994 The new cucumber variety of good quality, resistance andhigh yield-JinChun-4China Vegetables213(in Chinese)
9. Ma C Jian G L Zheng C L Chen Q Y Li C X Wei Q C Liu S C 1992 Initial studiesof suppressive soil of Fusarium oxysporum f. sp. vasinpectum (ATK)Cotton Science4(2)7783(in Chinese)
10. Wang B L 2000 Complete Produce Technique for Southern Greenhouse VegetablesBeijingChinaAgricultural Press120(in Chinese)
11. Wang L G Shen A L Sun K G Wu J C 2001 Research advances on obstacles and modulations of soybean continuouscroppingSoils and Fertilizers538(in Chinese)
12. Wang Q Li X L 2003 Effects ofbenzoic and cinnamic acids on watermelon seedling growth and fusariumwilt occurrenceJournal of China AgriculturalUniversity8(1)8386(in Chinese)
13. Wu F Z Meng L J Wen J Z 2002 Effect of cucumber exudates on the mycelialgrowth of Fusarium wiltChina Vegetables52627(in Chinese)
14. Yang Z W Wang R X Zong Z F Wang S M Wang C J 1995 Initial studies of pathogensuppressive soil to Fusarium wilt of cotton. І. Effects of rooteffusion of cotton on the Fusarium oxysporum f. sp. vasinfectumActa Agriculturae Boreal-occidentails Sinica4(4)6368(in Chinese)
15. Yu J Q Matsui Y 1994 Phytotoxicsubstances in root exudates of cucumberJournal of Chemical Ecology202130. doi:10.1007/BF02065988
16. Yu J Q Matsui Y 1997 Effects ofroot exudates of cucumber (Cucumis sativus L.) and allelochemicals on ion uptake by cucumber seedlingJournal of Chemical Ecology23817827. doi:10.1023/B:JOEC.0000006413.98507.55
17. Yuan H X Li H L Wang Y Fang W P Wang Z Y 2002 The root exudates of cottoncultivars with the different resistance and their effects on Verticillium dahliaeActa Phytopathologica Sinica32(2)127131(in Chinese)
18. Zhang F L Zhou B L Wang R H He Y 2005 Allelopathic effects of grafted eggplant root exudatesChinese Journal of Applied Ecology16(4)750753(in Chinese)
19. Zhen W C Wang X Y Cao K Q Jin Z J 2004 Study on determination and allelopathy of amino acids in strawberryroot exudates and decomposing productsJournalof Agricultural University of Hebei27(2)7680(in Chinese)
20. Zhou Z H Luo S M Mou Z P 1997 Allelopathic effect of tomatoChinese Journal of Applied Ecology8(4)445449(in Chinese)
21. Zou Q 2000 Experimental Guidance of Plant PhysiologyBeijingChina Agricultural Press60–63159160(in Chinese)