Active oxygen metabolism in the floral buds and leaves of the new cytoplasm male sterile (CMS) line and its maintainer line of non-heading Chinese cabbage

Abstract

The cytoplasm male sterile (CMS) line and its maintainer line of the newly-bred non-heading Chinese cabbage were taken as experimental materials. The production rate of superoxide anion radical (O_2^{-} ), contents of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂), and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) as well as ascorbate peroxidase (APX) were determined in buds of different developmental stages and leaves during vegetative and florescence stages. As showed by the results, there were higher levels of MDA and H2O2 contents, higher O_2^{-} production rate and higher activities of SOD, POD, and CAT in the buds of the CMS lines than that in the buds of the fertile lines, while the APX activity was different from the other three enzymes above. There were differences in activities of protective enzymes in leaves but no significant differences in MDA contents and O_2^{-} production rate, which showed that the differences in metabolism of active oxygen between CMS and its maintainer were mainly apparent in reproductive organs.

Keywords

non-heading Chinese cabbage / cytoplasm male sterility (CMS) / lipid peroxdation / active oxygen metabolism / protective enzymes

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2007 Higher Education Press