Advances in microbial mevalonolactone production: from fermentative mevalonate accumulation to downstream lactonization

Abstract

Mevalonolactone (MVAL) is a high-value feedstock for the cosmetic industry, with (R)-(-)-MVAL as the sole bioactive enantiomer. Chemical synthesis, which is the traditional method for MVAL production, is hindered by cumbersome procedures, low chiral purity, and sensitivity to humidity. Microbial fermentation via fermentative mevalonate (MVA) accumulation followed by in vitro acid-catalyzed lactonization has emerged as a promising alternative for producing optically pure (R)-(-)-MVAL. Strategies for MVA overproduction in microbial systems are reviewed, including the selection of chassis strains and enzymes for the MVA biosynthetic pathway, metabolic engineering approaches for strain improvement, optimization of fermentation processes, and downstream processes for MVA-to-MVAL lactonization. Finally, prospects for advancing microbial MVAL production are discussed.

Keywords

Mevalonolactone / Microbial cell factories / Biosynthesis / Metabolic engineering / Mevalonate lactonization

CRediT authorship contribution statement

Hao Tang: Writing - original draft, Visualization, Conceptualization. Lihong Yin: Writing - original draft. Yiwen Jiang: Writing - review & editing, Conceptualization. Teng Xu: Writing - review & editing. Ting Xue: Writing - review & editing. Meimei Fu: Writing - review & editing. Jianghao Chen: Writing - review & editing. Jinshan Guo: Writing - review & editing, Writing - original draft.

Declaration of competing interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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