Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system

Abstract

A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be Acinetobacter pittii, with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.

Keywords

Acinetobacter pittii / Microcrystalline cellulose / Cellulose nanoparticle / Controlled-release drug delivery system / Doxorubicin

Data Availability Statement

All data generated or analyzed during this study are included in this published article and its supplementary information files or are available upon request.

Declaration of Competing Interest

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:

Omer Faruk Sarioglu reports financial support was provided by Scientific and Technological Research Council of Turkey. Ruken Sariboga reports financial support was provided by Scientific and Technological Research Council of Turkey. Omer Faruk Sarioglu reports a relationship with Scientific and Technological Research Council of Turkey that includes: funding grants. Ruken Sariboga reports a relationship with Scientific and Technological Research Council of Turkey that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

CRediT authorship contribution statement

Ruken Sariboga: Writing - review & editing, Writing - original draft, Methodology, Investigation. Omer Faruk Sarioglu: Writing - review & editing, Writing - original draft, Validation, Supervision.

Acknowledgments

This study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK 123S091). The authors thank Istanbul Medeniyet University Science and Advanced Technologies Research Center (BILTAM) for providing the environment for conducting the research and Bogazici University Life Sciences and Technologies Application and Research Center (LifeSci) for assistance with SEM imaging and FTIR spectroscopy analysis. The authors also thank Prof. Dr. Murat Kazanci for fruitful discussions regarding the evaluation of the FTIR spectroscopy data.

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