Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications

Abstract

The biotechnological industry faces a crucial demand for novel bioactive compounds, particularly antimicrobial agents, to address the rising challenge of bacterial resistance to current available antibiotics. Traditional strategies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial producers. This study presents a protocol for targeted selection of bacterial strains using the supernatant of Paenibacillus elgii, which produces abundant signal molecules and antimicrobial peptides. Soil samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant, indicating their potential to produce similar compounds. The bacterial strains isolated through this method were assessed for their antibacterial activity. In addition, the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological interest. This study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.

Keywords

Prospecting / Supernatant / Antibacterial activity

Data Availability Statement

The type strain is K003, which is isolated from the soil of the Brazilian savanna-like Cerrado biome, Brasilia, Brazil. The deposit of the K003 genome in the GenBank database is being processed under accession number JAZBNP000000000.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

CRediT authorship contribution statement

I. C. Cunha-Ferreira: Writing - original draft, Investigation, Formal analysis, Data curation, Conceptualization. C. S. Vizzotto: Investigation, Formal analysis, Data curation, Conceptualization. T. D. Frederico: Formal analysis, Data curation. J. Peixoto: Writing - review & editing, Writing - original draft, Formal analysis, Data curation. L. S Carvalho: Writing - review & editing, Methodology. M. R. Tótola: Writing - review & editing, Methodology, Investigation, Formal analysis, Data curation. R. H. Krüger: Writing - review & editing, Writing - original draft, Validation, Supervision, Resources, Project administration, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation, Conceptualization.

Acknowledgments

The authors thank the Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente (Universidade Federal de Viçosa - UFV) for the infrastructure to carry out part of the experiments in this work. This study was funded by the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), and the Federal District Research Support Foundation (FAP-DF).

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