Assessment of proximate composition and antinutrients of bean varieties grown in stony soils in Southeastern Mexico

Abstract

Aim: The common bean (Phaseolusvulgaris L.) is a vital source of protein, dietary fiber, minerals, and bioactive compounds in rural and low-income populations. The objective of this study was to evaluate the proximate composition, antinutritional factors, and cooking quality of four black bean varieties (Jamapa, Grijalva, Tacaná, and INIFAP) grown in stony soils of Southeastern Mexico. Methods: Moisture, crude protein, crude fat, ash, crude fiber, and total carbohydrates were determined. The antinutritional factors assessed included cyanogenic glycosides, tannins, trypsin inhibitors, and phytates. Cooking quality was evaluated via cooking time and cooked grain hardness. All determinations were made in triplicate, and statistical differences among varieties were evaluated by one-way ANOVA with Tukey’s honestly significant difference (HSD) post-hoc test (p<0.05). Results: Significant differences were found between varieties (p<0.05), with Jamapa and Grijalva standing out for their higher protein content (~27.5%) and, along INIFAP, for their low trypsin inhibitor content (~4 ITU/mg), while their phytate levels ranged between 46 and 65 mg/g. All antinutritional factors were found within acceptable ranges for human consumption. Regarding technological properties, the Tacaná variety exhibited the shortest cooking time (25.3 min) and cooked grain hardness (1.86 N), making it an attractive option for consumers and the food industry. Conclusions: The evaluated bean varieties showed favorable nutritional profiles, low antinutrient concentrations, and superior cooking performance, rendering them suitable for human consumption. Their successful adaptation to stony-soil conditions underscores their potential to enhance agricultural and nutritional resilience in marginal environments.

Keywords

common bean / antinutritional factors / chemical composition / cooking time / resilient crops

References

[1] Murube E, Beleggia R, Pacetti D, Nartea A, Frascarelli G, Lanzavecchia G, et al. Characterization of Nutritional Quality Traits of a Common Bean Germplasm Collection. Foods. 2021;10:1572.

[2] Godfray HCJ, Aveyard P, Garnett T, Hall JW, Key TJ, Lorimer J, et al. Meat consumption, health, and the environment. Science. 2018;361:eaam5324.

[3] Foyer CH, Lam HM, Nguyen HT, Siddique KH, Varshney RK, Colmer TD, et al. Neglecting legumes has compromised human health and sustainable food production. Nat Plants. 2016;2:16112.

[4] Alvarado—López AN, Gómez—Oliván LM, Heredia JB, Baeza—Jiménez R, Garcia—Galindo HS, Lopez—Martinez LX. Nutritional and bioactive characteristics of Ayocote bean (Phaseoluscoccienus L.): An underutilized legume harvested in Mexico . CyTA J Food. 2019;17:199-206.

[5] Ramírez—Jiménez AK, Gaytán—Martínez M, Morales—Sánchez E, Loarca—Piña G. Functional properties and sensory value of snack bars added with common bean flour as a source of bioactive compounds. LWT. 2018;89:674-80.

[6] Das G, Sharma A, Sarkar PK. Conventional and emerging processing techniques for the post—harvest reduction of antinutrients in edible legumes. Appl Food Res. 2022;2:100112.

[7] Bessada SMF, Barreira JCM, Oliveira MBPP. Pulses and food security: Dietary protein, digestibility, bioactive and functional properties. Trends Food Sci Technol. 2019;93:53-68.

[8] Cominelli E, Sparvoli F, Lisciani S, Forti C, Camilli E, Ferrari M, et al. Antinutritional factors, nutritional improvement, and future food use of common beans: A perspective. Front Plant Sci. 2022;13:992169.

[9] Geleta RJ, Roro AG, Terfa MT. Phenotypic and yield responses of common bean (Phaseolus vulgaris l.) varieties to different soil moisture levels. BMC Plant Biol. 2024;24:242.

[10] Ramírez—Jaramillo G, Lozano—Contreras MG, Silva JHR. Agroclimatic Suitability ofPhaseolusvulgaris L., in the Yucatan Peninsula of Mexico . OALib. 2023;10:1-11.

[11] Pena FB, Silva AA, Theodoro JMV, Santos MB, de Carvalho CWP, Martino HSD, da Silva BP. Physicochemical, nutritional, and sensory properties of ready—to—eat whole grain bean snacks. Food Res Int. 2025;217:116754.

[12] Schad P. World Reference Base for Soil Resources—Its fourth edition and its history. J Plant Nut Soil Sci. 2023;186:151-63.

[13] Official Methods of Analysis, 22nd Edition (2023)[Internet]. AOAC International; c2023 [cited 2025 Dec 15]. Available from: https://www.aoac.org/official—methods—of—analysis/

[14] Roberts—Nkrumah LB. Breadnut and breadfruit propagation[Internet]. FAO; c2012 [cited 2025 Dec 10]. Available from: https://www.fao.org/3/a—i3085e.pdf

[15] da Silva Pereira ML, de Cássia Paulino de Souza R, de Medeiros JVF, de Brito GQ, Schwarz A. Determination of cyanide in whole seeds and brans of linseed (Linumusitatissimum Linn) by molecular spectrophotometry . Braz J Pharm Sci. 2023;59:e23059.

[16] Hasanaklou HT, Pipan B, Meglič V, Nagl N, Sinkovič L. Trypsin inhibitors in seeds and pods ofPhaseolusvulgaris/coccineus: A comparative study of shaking and ultrasonic extraction methods . Electron J Biotechnol. 2024;71:47-56.

[17] Wang Y, Li J, Wang B, Zhang Y, Geng J, Wen LX, et al. Effective Quantification of Tannin Content in Sorghum Grains Using Near—infrared Spectroscopy. IJAST. 2021;5:7.

[18] Siroli L, Giordani B, Rossi S, Gottardi D, McMahon H, Augustyniak A, et al. Antioxidant and Functional Features of Pre—Fermented Ingredients Obtained by the Fermentation of Milling By—Products. Fermentation. 2022;8:722.

[19] Ferreira CD, Ziegler V, Paraginski RT, Vanier NL, Elias MC, Oliveira M. Physicochemical, antioxidant and cooking quality properties of long—term stored black beans: effects of moisture content and storage temperature. Int Food Res J. 2017;24:2490—9.

[20] NMX—FF—038—SCFI—2016. Productos alimenticios no industrializados para consumo humano—fabáceas—frijol (Phaseolusvulgaris L.). Especificaciones y métodos de prueba . 2020.

[21] Lenora SE, Romelle FD, Flore DTNE, Flavie MKC, Alix MN, Bernard T. Physical and nutritional properties of sixteen varieties of bean seeds (Phaseolusvulgaris L.) grown in Cameroon . Discov Food. 2025;192:5.

[22] Celmeli T, Sari H, Canci H, Sari D, Adak A, Eker T, et al. The Nutritional Content of Common Bean (Phaseolusvulgaris L.) Landraces in Comparison to Modern Varieties . Agronomy. 2018;8:166.

[23] Amongi W, Aparicio J, Nduwarugira E, Ndabashinze B, Ruhebuza K, Otsyula R, et al. Yield and climatic parameters in a common bean (Phaseolusvulgaris L.) regional trial . Crop Sci. 2025;65:e70037.

[24] Rodríguez Madrera R, Campa Negrillo A, Ferreira Fernández JJ. Modulation of the nutritional and functional values of common bean by farming system: organic vs. conventional. Front Sustain Food Syst. 2024;7:1282427.

[25] Darkwa K, Ambachew D, Mohammed H, Asfaw A, Blair MW. Evaluation of common bean (Phaseolusvulgaris L.) genotypes for drought stress adaptation in Ethiopia . Crop J. 2016;4:367-76.

[26] Sinkovič L, Blažica V, Blažica B, Meglič V, Pipan B. How Nutritious Are French Beans (Phaseolusvulgaris L.) from the Citizen Science Experiment? Plants (Basel). 2024;13:314.

[27] Uebersax MA, Cichy KA, Gomez FE, Porch TG, Heitholt J, Osorno JM, et al. Dry beans (Phaseolusvulgaris L.) as a vital component of sustainable agriculture and food security—A review . Legume Sci. 2023;5:e155.

[28] López E, Becerra N, Cano O, Zaleta D, Acosta J. Adaptation and the technological quality of the “Negro Tacana” bean variety. Agron Mesoam. 2016;7:26-34.

[29] Perucini—Avendaño M, Arzate—Vázquez I, De Jesús Perea—Flores M, Tapia—Maruri D, Méndez—Méndez JV, Nicolás—García M, et al. Effect of cooking on structural changes in the common black bean (Phaseolusvulgaris var. Jamapa) . Heliyon. 2024;10:e25620.

[30] Semba RD, Ramsing R, Rahman N, Kraemer K, Bloem MW. Legumes as a sustainable source of protein in human diets. Global Food Security. 2021;28:100520.

[31] Chávez—Mendoza C, Sánchez E. Bioactive Compounds from Mexican Varieties of the Common Bean (Phaseolus vulgaris): Implications for Health. Molecules. 2017;22:1360.

[32] Lisciani S, Marconi S, Le Donne C, Camilli E, Aguzzi A, Gabrielli P, et al. Legumes and common beans in sustainable diets: nutritional quality, environmental benefits, spread and use in food preparations. Front Nutr. 2024;11:1385232.

[33] Rocha L, Oliveira L, Dalvi L, Oliveira F. Soil fertility and genotype affect yield components and mineral grain concentration of common beans (Phaseolus vulgaris L.). Trop Subtrop Agroecosystems. 2020;23:87.

[34] Sedov S, Rivera—Uria MY, Ibarra—Arzave G, García—Ramírez P, Solleiro—Rebolledo E, Cabadas—Báez HV, et al. Soil toposequences, soil erosion, and ancient Maya land use adaptations to pedodiversity in the tropical karstic landscapes of southern Mexico. Front Earth Sci. 2023;11:1239301.

[35] Chamoun D, Duncan AM, Lukus PK, Loreto MD, Pals—Horne F, Hawke A, et al. Postprandial Blood Glucose and Insulin Response in Healthy Adults When Lentils Replace High—Glycemic Index Food Ingredients in Muffins, Chilies and Soups. Nutrients. 2024;16:2669.

[36] Palupi H, Estiasih T, Yunianta, Sutrisno A. Physicochemical and protein characterization of lima bean (Phaseoluslunatus L) seed . Food Res. 2022;6:168-77.

[37] Niyogi A, Bhattacharyya S, Pal S, Mukherjee S, Sarkar S, Ghosh K, et al. A comprehensive overview of cyanogenic glycosides: Toxicology and innovative sensing approaches. Food Control. 2025;177:111431.

[38] Jiménez—González C, Fernández—De—Castro L, Torrado AM, Fuciños C, Díaz—de—Apodaca E, Rúa ML. Nutritional evaluation of high—value alternative proteins extracted from legume defective seeds. Food Chem. 2025;481:143936.

[39] Bai Q, Li M, Zhou J, Imran A, de Souza TSP, Barrow C, et al. Influence of Processing Methods on Phytochemical Composition of Different Varieties of Beans (Phaseolusvulgaris) . Food Rev Int. 2023;40:1941-79.

[40] Corzo—Ríos LJ, Sánchez—Chino XM, Cardador—Martínez A, Martínez—Herrera J, Jiménez—Martínez C. Effect of cooking on nutritional and non—nutritional compounds in two species of Phaseolus (P.vulgaris andP.coccineus) cultivated in Mexico . Int J Gastron Food Sci. 2020;20:100206.

[41] Ojo MA. Tannins in Foods: Nutritional Implications and Processing Effects of Hydrothermal Techniques on Underutilized Hard—to—Cook Legume Seeds—A Review. Prev Nutr Food Sci. 2022;27:14-9.

[42] Nagessa WB, Chambal B, Macuamule C. Effects of processing methods on phytate and tannin content of black small common beans (Phaseolusvulgaris L.) cultivated in Mozambique . Cogent Food Agric. 2023;9:2289713.

[43] Tafiire H, Wainaina IN, Lugumira R, An NT, Ogwok P, Grauwet T, et al. A detailed study on the cooking kinetics of fresh and hard to cook common beans (Phaseolusvulgaris L.): A case study on bean accessions of different market classes . J Food Eng. 2024;381:112186.

[44] Sánchez—Arteaga H, Urías—Silvas JE, Espinosa—Andrews H, García—Márquez E. Effect of chemical composition and thermal properties on the cooking quality of common beans (Phaseolusvulgaris) . CyTA J Food. 2014;13:385-91.

[45] Purwandari FA, Annisa EDN, Rachmawati AT, Puspitasari D, Wikandari R, Seyaningsih W, et al. Effect of different cooking methods on chemical composition, nutritional values, and sensory properties of Jack bean (Canavaliaensiformis) tempe . Food Res. 2021;5:327—33.

[46] Diaz S, Ariza—Suarez D, Ramdeen R, Aparicio J, Arunachalam N, Hernandez C, et al. Genetic Architecture and Genomic Prediction of Cooking Time in Common Bean (Phaseolusvulgaris L.) . Front Plant Sci. 2021;11:622213.

[47] López—Hernández F, Villanueva—Mejía DF, Tofiño—Rivera AP, Cortés AJ. Genomic Prediction of Adaptation in Common Bean (Phaseolus vulgaris L.) × Tepary Bean (P. acutifolius A. Gray) Hybrids. Int J Mol Sci. 2025;26:7370.