Characterization of carotenoids and color in temperate Asimina triloba and comparison to other tropical Annonaceae fruits

Abstract

Aim: Fruits from the tropical Annona genus of family Annonaceae have long been cultivated in tropical Latin America, Africa, and southeastern Asia. Asimina triloba is a temperate fruit from Annonaceae, but few comparisons between Annonaceae fruits exist. The objective was to determine how 21 days of refrigerated storage affected the carotenoids and color in ripe and overripe A. triloba. A comparison to tropical Annonaceae fruits is provided. Methods: Pawpaw pulp was stored refrigerated for 21 days. Total carotenoids and β-carotene were determined spectrophotometrically and by HPLC, respectively. C.I.E. L*, a*, and b* values were used to calculate hue angle, chroma, total color change (ΔE), browning index, which have been reported in A. triloba previously, and color index, whiteness index, yellowness index, the ratio of a*/b*, and percent change in L*, which are reported herein for the first time. Results: Overripe pulp contains 5-fold more carotenoids than ripe pulp. A significant decline in total carotenoids was observed during refrigerated storage in the overripe pulp, but not in fresh pulp. At the onset of refrigerated storage, ripe pulp was significantly less brown than overripe pulp, but became more brown during refrigerated storage. No further change in browning was observed during storage of overripe pulp. Conclusions: Using established conversion factors and the values generated in this study, preliminary indications are that ripe A. triloba pulp provides 4.0% (males) and 5.1% (females) of the U.S. Recommended Dietary Allowance (USRDA) of vitamin A for individuals 14 years or older, and overripe provides 3.5% and 4.6%. Carotenoids are well-characterized for Annona muricata (A. muricata), Annona reticulata (A. reticulata), and Annona squamosa (A. squamosa), but these provide less than 1% of the USRDA of vitamin A. A comparison revealed that Annonaceae fruit are nutrient-dense, provide fiber and potassium, are low in fat and protein, and have comparable calcium, iron, magnesium, potassium, and sodium levels. Understanding Annonaceae fruits nutritional value may facilitate increased economic potential.

Keywords

Annonaceae / Annona / Asimina / nutritional comparison / carotenoids

References

[1] Hernández Fuentes LM, Montalvo González E, García Magaña ML, Anaya Esparza LM, Nolasco González Y, Villagrán Z, et al. Current Situation and Perspectives of Fruit Annonaceae in Mexico: Biological and Agronomic Importance and Bioactive Properties. Plants (Basel). 2021; 11: 7. [DOI] [PubMed] [PMC]

[2] Paull RE, Duarte O. Annonas: cherimoya, atemoya and sweetsop. In: Tropical fruits. 2nd ed. Wallingford: CABI; 2011. pp. 123—52.

[3] Greenawalt LM, Powell R, Simon J, Brannan RG. A Retrospective Analysis of Pawpaw (Asimina triloba [L.] Dunal) Production Data from 2005—2012 . J Am Pomol Soc. 2019; 73: 2-11.

[4] Janzen DH, Martin PS. Neotropical Anachronisms: The Fruits the Gomphotheres Ate. Science. 1982; 215: 19-27. [DOI] [PubMed]

[5] Brannan RG, Coyle MN. Worldwide Introduction of North American Pawpaw (Asimina triloba): Evidence Based on Scientific Reports. Sustain Agric Res. 2021; 10: 19-24. [DOI]

[6] Maoka T. Carotenoids as natural functional pigments. J Nat Med. 2020; 74: 1-16. [DOI] [PubMed] [PMC]

[7] Elvira—Torales LI, García—Alonso J, Periago—Castón MJ. Nutritional Importance of Carotenoids and Their Effect on Liver Health: A Review. Antioxidants (Basel). 2019; 8: 229. [DOI] [PubMed] [PMC]

[8] Burri BJ, La Frano MR, Zhu C. Absorption, metabolism, and functions of β—cryptoxanthin. Nutr Rev. 2016; 74: 69-82. [DOI] [PubMed] [PMC]

[9] Mitra S, Rauf A, Tareq AM, Jahan S, Emran TB, Shahriar TG, et al. Potential health benefits of carotenoid lutein: An updated review. Food Chem Toxicol. 2021; 154: 112328. [DOI] [PubMed]

[10] Sharma I, Khare N, Rai A. Carotenoids: Sources, Bioavailability and Their Role in Human Nutrition. In: Dietary Carotenoids — Sources, Properties, and Role in Human Health. IntechOpen; 2024. [DOI]

[11] Khan UM, Sevindik M, Zarrabi A, Nami M, Ozdemir B, Kaplan DN, et al. Lycopene: Food Sources, Biological Activities, and Human Health Benefits. Oxid Med Cell Longev. 2021; 2021: 2713511. [DOI] [PubMed] [PMC]

[12] Pathare PB, Opara UL, Al—Said FAJ. Colour Measurement and Analysis in Fresh and Processed Foods: A Review. Food Bioprocess Technol. 2013; 6: 36-60. [DOI]

[13] Pomper KW, Layne DR, Peterson RN. The Pawpaw Regional Variety Trial. In: Janick J, editor. Perspect New Crops New Uses. ASHS Press; 1999. pp. 353-7.

[14] Wang Y, Hao Y, Zhou D, Pan L, Tu K. Differences in commercial quality and carotenoids profile of yellow— and white—fleshed nectarine fruit during low temperature storage and the regulation of carotenoids by sugar. Postharvest Biol Technol. 2023; 197: 112206. [DOI]

[15] Brannan RG. Polyphenol oxidase in pawpaw (Asimina triloba Dunal) fruit pulp from different varieties . J Food Res. 2016; 5: 33-9. [DOI]

[16] Brannan RG, Peters T, Talcott ST. Phytochemical analysis of ten varieties of pawpaw (Asimina triloba [L.] Dunal) fruit pulp . Food Chem. 2015; 168: 656—61. [DOI] [PubMed]

[17] Vitamin A and Carotenoids[Internet]. National Institutes of Health; [cited 2025 Feb 1]. Available from: https://ods.od.nih.gov/factsheets/VitaminA—HealthProfessional/

[18] Brannan RG, Anderson EE, Powell RL, Coyle MN. A comparative analysis of pawpaw (Asimina triloba) quality and nutritional data . J Appl Bot Food Qual. 2021; 94: 131. [DOI]

[19] Donato P, Cacciola F, Mondello L, Giuffrida D, Beccaria M, Dugo P, et al. Analysis of the Carotenoid Composition and Stability in Various Overripe Fruits by Comprehensive Two—Dimensional Liquid Chromatography. LCGC Asia Pacific. 2016; 19: 6-12.

[20] Lokesh V, Divya P, Puthusseri B, Manjunatha G, Neelwarne B. Profiles of carotenoids during post—climacteric ripening of some important cultivars of banana and development of a dry product from a high carotenoid yielding variety. Food Sci Technol. 2014; 55: 59-66. [DOI]

[21] Sampaio SA, Bora PS, Holschuh HJ, Silva S de M. Postharvest respiratory activity and changes in some chemical constituents during maturation of yellow mombin (Spondias mombin) fruit. Food Sci Technol. 2007; 27: 511—5. [DOI]

[22] Hu K, Peng D, Wang L, Liu H, Xie B, Sun Z. Effect of mild high hydrostatic pressure treatments on physiological and physicochemical characteristics and carotenoid biosynthesis in postharvest mango. Postharvest Biol Technol. 2021; 172: 111381. [DOI]

[23] de Morais Cardoso L, da Silva Oliveira D, de Freitas Bedetti S, Martino HSD, Pinheiro—Sant’Ana HM. Araticum (Annona crassiflora Mart.) from the Brazilian Cerrado: chemical composition and bioactive compounds . Fruits. 2013; 68: 121-34. [DOI]

[24] Chimbevo ML, Malala JB, Anjili CO, Orwa J, Mibei EK, Ndeti CM, et al. Annonaceae Fruits Growing in Coast Region of Kenya as an Alternative Source of Dietary Carotenoids . Int J Food Sci Biotech. 2017; 2: 114-20. [DOI]

[25] Agricultural Research Service FoodData Central[Internet]. U.S. Department of Agriculture (USDA); [cited 2025 Feb 1]. Available from: https://fdc.nal.usda.gov/

[26] Isabelle M, Lee BL, Lim MT, Koh WP, Huang D, Ong CN. Antioxidant activity and profiles of common fruits in Singapore. Food Chem. 2010; 123: 77-84. [DOI]

[27] Albuquerque TG, Santos F, Sanches—Silva A, Oliveira MB, Bento AC, Costa HS. Nutritional and phytochemical composition of Annona cherimola Mill. fruits and by—products: Potential health benefits . Food Chem. 2016; 193: 187-95. [DOI] [PubMed]

[28] Gentile C, Mannino G, Palazzolo E, Gianguzzi G, Perrone A, Serio G, et al. Pomological, Sensorial, Nutritional and Nutraceutical Profile of Seven Cultivars of Cherimoya (Annona cherimola Mill) . Foods. 2020; 10: 35. [DOI] [PubMed] [PMC]

[29] Murillo E, Meléndez—Martínez AJ, Portugal F. Screening of vegetables and fruits from Panama for rich sources of lutein and zeaxanthin. Food Chem. 2010; 122: 167-72. [DOI]

[30] Nam JS, Jang HL, Rhee YH. Nutritional compositions in roots, twigs, leaves, fruit pulp, and seeds from pawpaw (Asimina triloba Dunal) grown in Korea . J Appl Bot Food Qual. 2018; 91: 47-55. [DOI]

[31] Schiassi MCEV, Souza VR, Lago AMT, Campos LG, Queiroz F. Fruits from the Brazilian Cerrado region: Physico—chemical characterization, bioactive compounds, antioxidant activities, and sensory evaluation. Food Chem. 2018; 245: 305—11. [DOI] [PubMed]

[32] Julián—Loaeza AP, Santos—Sánchez NF, Valadez—Blanco R, Sánchez—Guzmán BS, Salas—Coronado R. Chemical composition, color, and antioxidant activity of three varieties of Annona diversifolia Safford fruits . Ind Crops Prod. 2011; 34: 1262-8. [DOI]

[33] Tran NYT, Nhan NPT, Thanh VT, Chinh ND, Tri DL, Nguyen DV, et al. Effect of storage condition on color, vitamin C content, polyphenol content and antioxidant activity in fresh soursop pulp (Annona muricata L.) . In: IOP Conference Series: Materials Science and Engineering. IOP Publishing; 2020. p. 022065. [DOI]

[34] Márquez Cardozo CJ, Villacorta Lozano V, Yepes Betancur DP, Ciro Velásquez HJ, Cartagena Valenzuela JR. Physiological and physico—chemical characterization of the soursop fruit (Annona muricata L. cv. Elita). Rev Fac Nac Agron Medellín. 2012; 65: 6477-86.

[35] Lolletti D, Principio L, Ciorba R, Mitrano F, Ceccarelli D, Antonucci F, et al. Asimina triloba: Crop years, cultivars and ripening time influence on qualitative parameters . Sci Hortic. 2021; 289: 110481. [DOI]

[36] Zhang L, Dai S, Brannan RG. Effect of high pressure processing, browning treatments, and refrigerated storage on sensory analysis, color, and polyphenol oxidase activity in pawpaw (Asimina triloba L.) pulp . LWT. 2017; 86: 49-54. [DOI]

[37] Adainoo B, Crowell B, Thomas AL, Lin CH, Cai Z, Byers P, et al. Physical characterization of frozen fruits from eight cultivars of the North American pawpaw (Asimina triloba) . Front Nutr. 2022; 9: 936192. [DOI] [PubMed] [PMC]

[38] Peterson RN, Cherry JP, Simmons JG. Composition of pawpaw (Asimina triloba) fruit . Annu Rep North Nut Grow Assoc. 1982.