Rapid identification of high-temperature Daqu Baijiu with the same aroma type by UV-VIS sensor of HBT combined with Zn²⁺

Abstract

Aim: Baijiu adulteration has always been a hot spot of social concern, especially high-temperature Daqu Baijiu, because of its better flavor quality and high value, it faces a challenge from illegal adulteration of high-grade Baijiu bottles with low-grade Baijiu. Methods: A new ultraviolet-visible spectroscopy (UV-VIS) sensor method based on 2-(2-hydroxyphenyl) benzothiazole (HBT) combined with Zn²⁺ was constructed. The specific mechanism of light signal change was mainly based on the competitive coordination effect of pyrazines and other nitrogen-containing compounds in high-temperature Daqu Baijiu and small molecular probe HBT on Zn²⁺ and the excited state intramolecular proton transfer (ESIPT) mechanism of HBT itself. Results: The random forest results showed that the prediction set classification accuracy was improved from 62.37% to 100%. The accuracy of the data driven-soft independent modelling of class analogies (DD-SIMCA) model was between 90% and 100%, indicating that the array sensor had a good recognition effect on the adulteration of the same aroma type of Baijiu. Twelve kinds of Maillard reaction products such as tetramethylpyrazine, 2,6-dimethylpyrazine, and furfural in Baijiu were predicted successfully, and the deviation between the real value and the predicted value was less than 1.8746% ± 1.4515%. Conclusions: This method not only improves the specificity of spectral detection, and the amount of information extracted from Baijiu samples, but also the detection time is shortened to less than 3 min, and the accurate identification of high-temperature Daqu Baijiu with the same aroma type was realized. This method can provide reliable help for the rapid identification of high-temperature Daqu and other precious Baijiu, even traditional fermented food.

Keywords

High-temperature Daqu Baijiu / 2-(2-hydroxyphenyl) benzothiazole / ultraviolet-visible spectroscopy / authenticity identification

References

[1] Tu W, Cao X, Cheng J, Li L, Zhang T, Wu Q, et al. Chinese Baijiu: The Perfect Works of Microorganisms. Front Microbiol. 2022; 13: 919044.

[2] Liu H, Sun B. Effect of Fermentation Processing on the Flavor of Baijiu. J Agric Food Chem. 2018; 66: 5425—32.

[3] Wang L, Chen S, Xu Y. Distilled beverage aging: A review on aroma characteristics, maturation mechanisms, and artificial aging techniques. Compr Rev Food Sci Food Saf. 2023; 22: 502-34.

[4] Hong JX, Zhao DR, Sun BG. Research progress on the profile of trace components in Baijiu. Food Rev Int. 2023; 39: 1666-93.

[5] Yi Z, Jin Y, Xiao Y, Chen L, Tan L, Du A, et al. Unraveling the Contribution of High Temperature Stage to Jiang—Flavor Daqu, a Liquor Starter for Production of Chinese Jiang—Flavor Baijiu, With Special Reference to Metatranscriptomics. Front Microbiol. 2019; 10: 472.

[6] Xiao C, Lu Z, Zhang X, Wang S, Ao L, Shen C, et al. Bio—Heat Is a Key Environmental Driver Shaping the Microbial Community of Medium—Temperature Daqu. Appl Environ Microbiol. 2017; 83: e01550—17.

[7] Wang M, Yang J, Zhao Q, Zhang K, Su C. Research Progress on Flavor Compounds and Microorganisms of Maotai Flavor Baijiu. J Food Sci. 2019; 84: 6-18.

[8] He X, Yangming H, Górska—Horczyczak E, Wierzbicka A, Jeleń HH. Rapid analysis of Baijiu volatile compounds fingerprint for their aroma and regional origin authenticity assessment. Food Chem. 2021; 337: 128002.

[9] Zong XY, Zhou XJ, Wen L, Gan S, Li L. Identification of Baijiu based on the Raman spectroscopy and back—propagation neural network optimized using genetic algorithm. J Food Compos Anal. 2023; 126: 105917.

[10] Jiang X, Xie Y, Wan D, Chen M, Zheng F. GUITAR—enhanced facile discrimination of aged Chinese Baijiu using electrochemical impedance spectroscopy. Anal Chim Acta. 2019; 1059: 36-41.

[11] Hu SQ, Wang L. Age discrimination of Chinese Baijiu based on midinfrared spectroscopy and chemometrics. J Food Quality. 2021; 2021: 5527826.

[12] Xu HB, Shao KY, Hu WX. Synthesis and Spectroscopy of 2—(2—Hydroxyphenyl) benzothiazole. Microwave Chem. 2020; 4: 13-21. Chinese.

[13] Weller A. Intramolecular proton transfer in excited states. Z Elektrochem Angew Phys Chem. 1956; 60: 1144—7. German.

[14] Hamada Y, Sano T, Fujii H, Nishio Y, Takahashi H, Shibata K. White—light—emitting material for organic electroluminescent devices. Jpn J Appl Phys. 1996; 35: L1339.

[15] Chen HY, Zhu YM, Xie YF, Long WJ, Lan W, She YB, et al. Rapid identification of high—temperature Daqu Baijiu with the same aroma type through the excitation emission matrix fluorescence of maillard reaction products. Food Control. 2023; 153: 109938.

[16] Azizan NI, Mokhtar NFK, Arshad S, Sharin SN, Mohamad N, Mustafa S, et al. Detection of lard adulteration in wheat biscuits using chemometrics—assisted GCMS and random forest. Food Anal Method. 2021; 14: 2276—87.

[17] Chung HT, Tsai CC, Jen KK, Huang YS, Ferng YC, Lo CY, et al. Optimization of process parameters of selective laser melted nickel—based superalloy for densification by random forest regression algorithm and response surface methodology. Results Eng. 2024; 22: 102182.

[18] Pagani AP, Camargo G, Ibañez GA, Olivieri AC, Pomerantsev AL, Rodionova OY. Data—Driven Version of Multiway Soft Independent Modeling of Class Analogy (N—Way DD—SIMCA): Theory and Application. Anal Chem. 2024; 96: 4845-53.

[19] Zhu Y, Xiang F, Su Y, Jiang X, Cang Y, Long W, et al. Authenticity identification of high—temperature Daqu Baijiu through multi—channel visual array sensor of organic dyes combined with smart phone App. Food Chem. 2024; 438: 137980.

[20] Onça L, Koljančić N, Furdíková K, Khvalbota L, Špánik I, Gomes AA. A digital image smartphone—based approach to Slovak Tokaj wine authentication chemometric assisted. Food Chem. 2024; 456: 140075.

[21] Reis—Pereira M, Mazivila SJ, Tavares F, Neves dos Santos F, Cunha M. Early plant disease diagnosis through handheld UV—Vis transmittance spectrometer with DD—SIMCA one—class classification and MCR—ALS bilinear decomposition. Smart Agric Technol. 2024; 9: 100631.

[22] Ju Y, Liu H, Niu S, Kang L, Ma L, Li A, et al. Optimizing geographical traceability models of Chinese Lycium barbarum: Investigating effects of region, cultivar, and harvest year on nutrients, bioactives, elements and stable isotope composition . Food Chem. 2024; 467: 142286.

[23] Kuligowski J, Moreno—Torres M, Quintás G. Improving insights from metabolomic functional analysis combining multivariate tools. Anal Chim Acta. 2024; 1323: 343062.

[24] Wang X, He M, Zheng J, Ma Y, Hou C, Hou D. Methodology and optimization research for discrimination of different brands of Baijiu based on multispectral techniques. Food Measure. 2024; 18: 7855-67.

[25] Nowak A, Janoszka B, Szumska M, Tyrpień—Golder K. FURFURAL, HYDROXYMETHYLFURFURAL AND FUROSINE AS MAILLARD REACTION MARKERS IN FRUIT BASED FOODS INCLUDING JAMS AND BABY FOOD. JMBFS. 2021; 11: e1384.

[26] Xu HY. Study on the relationship between furfural content and flavor type in Baijiu. Liquor Making. 2002; 29: 37-9.

[27] Zhu XQ, Lei WH, Huang YY. Research progress of 5—hydroxymethyl furfural in food and its safety. J Food Saf Qual. 2022; 15: 4983-991.

[28] Li L. A preliminary study on the characteristics of Maotai Baijiu that cannot be copied. Changjiang Series. 2019; 89-90.

[29] Niu J, Yang S, Shen Y, Cheng W, Li H, Sun J, et al. What are the main factors that affect the flavor of sauce—aroma Baijiu. Foods. 2022; 11: 3534.

[30] Ling Y, Li W, Tong T, Li Z, Li Q, Bai Z, et al. Assessing the Microbial Communities in Four Different Daqus by Using PCR—DGGE, PLFA, and Biolog Analyses. Pol J Microbiol. 2020; 27-37.

[31] Wang AH, Zhang H, Abou—Zied OK, Yu C, Romesberg FE, Glasbeek M. Femtosecond fluorescence upconversion studies of excited—state proton—transfer dynamics in 2—(2′—hydroxyphenyl)benzoxazole (HBO) in liquid solution and DNA. Chem Phys Lett. 2003; 367: 599-608.

[32] Zheng J, Zhang G, Wu F, Ma L, Chen W. The spectra properties of 2—(2'—hydroxyphenyl)benzothiazole in different solvents. Guang Pu Xue Yu Guang Pu Fen Xi. 2008; 28: 970—3.