Metals and metalloid in medicinal plants: occurrence and risk assessment to human health

Abstract

Aim: This study was aimed at determining the levels of trace elements in six medicinal plants of tropical origin. Methods: The levels of As, Cd, Co, Cu, Fe, Mn, Ni, Pb, and Zn in Albizia glaberrima (AG), Aristolochia ringens (AR), Brysocarpus coccineus (BC), Ipomoea asarifolia (IA), Sansevieria liberica (SL), and Telfairia occidentalis (TO) were determined using an inductively coupled plasma-mass spectrometry. The estimated dietary intakes of the metals, hazard quotients (HQ), and hazard index (HI) were calculated. Results: The highest levels of Cd, Pb, Zn, and Fe were detected in IA. BC had the highest levels of Mn and Ni while AR had the highest levels of Cu, Co, and As. However, the levels of the metals were mostly below the permissible limits in the plants. The estimated dietary weekly intakes (EWIs) were below the provisional tolerable weekly intake for each chemical element. The EWIs range values were 21.566–643.114 µg/kg per day (kg is the unit of body weight), 0.008–1.529 µg/kg per day, 0.6–7.815 µg/kg per day, 67.569–215.889 µg/kg per day, 4.305–185.451 µg/kg per day, 0.225–1.704 µg/kg per day, 1.03–10.2 µg/kg per day, 0.933–2.286 µg/kg per day, and 62.554–854.4 µg/kg per day for Cu, Cd, Pb, Zn, Mn, Co, Ni, As, and Fe, respectively. The HQ values of the elements were less than 1 except for Cu in AR (1.321). The values of lifetime cancer risks exceeded the permissible limit in all the plant materials. Conclusions: The findings from the study revealed that the consumption of TO, SL, and AG for medicinal purposes has no inherent non-carcinogenic toxicity while the consumption of AR, IA, and BC has some risks of non-carcinogenic toxic. However, the six plant materials showed inherent risks of carcinogenic events, as such their use for medicinal purposes must be cautious, maybe by reducing both the ingestion rate and the frequency of intake.

Keywords

Traditional medicine / medicinal plants / toxic elements contamination / health risk assessment / inductively coupled plasma-mass spectrometry

References

[1] Kasilo OMJ, Wambebe C, Nikiema JB, Nabyonga—Orem J. Towards universal health coverage: advancing the development and use of traditional medicines in Africa. BMJ Glob Health. 2019; 4: e001517.

[2] Khan MSA, Ahmad I. Herbal medicine: current trends and future prospects. In: Khan MSA, Ahmad I, Chattopadhyay D, editors. New look to phytomedicine. Florida: Academic Press; 2019. pp. 3-13.

[3] Anand U, Jacobo—Herrera N, Altemimi A, Lakhssassi N. A comprehensive review on medicinal plants as antimicrobial therapeutics: potential avenues of biocompatible drug discovery. Metabolites. 2019; 9: 258.

[4] Süntar I. Importance of ethnopharmacological studies in drug discovery: role of medicinal plants. Phytochem Rev. 2020; 19: 1199-209.

[5] Lezoul NE, Belkadi M, Habibi F, Guillén F. Extraction processes with several solvents on total bioactive compounds in different organs of three medicinal plants. Molecules. 2020; 25: 4672.

[6] Moyo M, Aremu AO, Van Staden J. Medicinal plants: an invaluable, dwindling resource in sub—Saharan Africa. J Ethnopharmacol. 2015; 174: 595-606.

[7] de Santana BF, Voeks RA, Funch LS. Ethnomedicinal survey of a maroon community in Brazil’s Atlantic tropical forest. J Ethnopharmacol. 2016; 181: 37-49.

[8] Bibi F, Abbas Z, Harun N, Perveen B, Bussmann RW. Indigenous knowledge and quantitative ethnobotany of the Tanawal area, Lesser Western Himalayas, Pakistan. Plos One. 2022; 17: e0263604.

[9] Bouyahya A, Belmehdi O, Benjouad A, El Hassani RA, Amzazi S, Dakka N, et al. Pharmacological properties and mechanism insights of Moroccan anticancer medicinal plants: what are the next steps? Ind Crops Prod. 2020; 147: 112198.

[10] Ma RH, Ni ZJ, Zhu YY, Thakur K, Zhang F, Zhang YY, et al. A recent update on the multifaceted health benefits associated with ginger and its bioactive components. Food Funct. 2021; 12: 519-42.

[11] Elahee SF, Mao H, Shen X. Traditional Indian medicine and traditional Chinese medicine: a comparative overview. Chin Med Cult. 2019; 2: 105-13.

[12] Salvilla RCM, Faller EM. Indian and Chinese traditional medicine on COVID—19: a review and perspective. Int J Sci Res Publ. 2021; 11: 77-81.

[13] Watt JM, Breyer—Brandwijk MG. The medicinal and poisonous plants of Southern and Eastern Africa. Berlin: Springer; 1962.

[14] Orabueze IC, Uzor SC, Ndiaye B, Uba D, Ota DA, Agbedahusi J. Antimalarial, antioxidant activities and chemoprofile of Sansevieria liberica Gerome and Labroy (Agavaceae) leaf extract. Adv Pharmacol Pharm Sci. 2021; 2021: 9053262.

[15] Akindele AJ, Wani ZA, Sharma S, Mahajan G, Satti NK, Adeyemi OO, et al. In vitro and in vivo anticancer activity of root extracts of Sansevieria liberica Gerome and Labroy (Agavaceae) . Evid Based Complementary Altern Med. 2015; 2015: 560404.

[16] Meira M, Silva EP, David JM, David JP. Review of the genus Ipomoea: traditional uses, chemistry and biological activities. Rev Bras Farmacogn. 2012; 22: 682-713.

[17] Akindele AJ, Eksioglu EA, Kwan JC, Adeyemi OO, Liu C, Luesch H, et al. Biological effects of Byrsocarpus coccineus in vitro . Pharm Biol. 2011; 49: 152-60.

[18] Ogbiti VM, Akindele AJ, Adeyemi OO. Analgesic, anti—inflammatory, and antipyretic activities of hydroethanolic stem bark extract of Albizia glaberrima . J Herbs Spices Med Plants. 2017; 23: 44-67.

[19] Akindele AJ, Oladimeji—Salami JA, Usuwah BA. Antinociceptive and anti—inflammatory activities of Telfairia occidentalis hydroethanolic leaf extract (Cucurbitaceae) . J Med Food. 2015; 18: 1157-63.

[20] Akindele AJ, Wani Z, Mahajan G, Sharma S, Aigbe FR, Satti N, et al. Anticancer activity of Aristolochia ringens Vahl. (Aristolochiaceae) . J Tradit Complement Med. 2014; 5: 35-41.

[21] Luo L, Wang B, Jiang J, Fitzgerald M, Huang Q, Yu Z, et al. Heavy metal contaminations in herbal medicines: determination, comprehensive risk assessments, and solutions. Front Pharmacol. 2021; 11: 595335.

[22] Sadhu A, Upadhyay P, Singh PK, Agrawal A, Ilango K, Karmakar D, et al. Quantitative analysis of heavy metals in medicinal plants collected from environmentally diverse locations in India for use in a novel phytopharmaceutical product. Environ Monitor Assess. 2015; 187: 542.

[23] Tschinkel PF, Melo ES, Pereira HS, Silva K, Arakaki DG, Lima NV, et al. The hazardous level of heavy metals in different medicinal plants and their decoctions in water: a public health problem in Brazil. BioMed Res Int. 2020; 2020: 1465051.

[24] Vinogradova N, Glukhov A, Chaplygin V, Kumar P, Mandzhieva S, Minkina T, et al. The content of heavy metals in medicinal plants in various environmental conditions: a review. Horticulturae. 2023; 9: 239.

[25] Rafati Rahimzadeh M, Rafati Rahimzadeh M, Kazemi S, Moghadamnia A. Cadmium toxicity and treatment: an update. Caspian J Intern Med. 2017; 8: 135-45.

[26] Rizvi A, Parveen S, Khan S, Naseem I. Nickel toxicology with reference to male molecular reproductive physiology. Reprod Biol. 2020; 20: 3-8.

[27] Sabir S, Akash MS, Fiayyaz F, Saleem U, Mehmood MH, Rehman K. Role of cadmium and arsenic as endocrine disruptors in the metabolism of carbohydrates: inserting the association into perspectives. Biomed Pharmacother. 2019; 114: 108802.

[28] Rahaman MS, Rahman MM, Mise N, Sikder MT, Ichihara G, Uddin MK, et al. Environmental arsenic exposure and its contribution to human diseases, toxicity mechanism and management. Environ Pollut. 2021; 289: 117940.

[29] Adeoye I, Etuk V. Prevalence, predictors and pregnancy outcomes of unprescribed and herbal medicine use in Ibadan, Nigeria. BMC Complement Med Ther. 2023; 23: 17.

[30] Usai J, Ekeocha Z, Byrn S, Clase K. Herbal medicines registration process for Zimbabwe overview of the process. BIRS Africa Technical Report Papers; 2021 Nov. Report No.: TR—BIRSAFRICA— 2021—8.

[31] Adeyemi JA, Adedire CO, Paulelli AC, Martins—Junior AC, Ileke KD, Barbosa Jr F. Levels and dietary intake of lead (Pb) and six essential elements in gari samples from Ondo States, Southwest Nigeria: a potential risk factor of health status. J Food Compost Anal. 2016; 45: 34-8.

[32] Kenny CR, Ring G, Sheehan A, Mc Auliffe MAP, Lucey B, Furey A. Novel metallomic profiling and noncarcinogenic risk assessment of botanical ingredients for use in herbal, phytopharmaceutical and dietary products using HR—ICP—SFMS. Sci Rep. 2022; 12: 17582.

[33] Geronimo ACR, Melo ESP, Silva KRN, Pereira HS, Nascimento VA, Machate DJ, et al. Human health risk assessment of heavy metals and metalloids in herbal medicines used to treat anxiety: monitoring of safety. Front Pharmacol. 2021; 12: 772928.

[34] Bakar MA, Bhattacherjy SC. Assessment of heavy metals concentration in some selected medicinal plants collected from BCSIR, Chittagong cultivation area in Bangladesh. Hamdard Med. 2012; 55: 26-32.

[35] Annan K, Dickson RA, Amponsah IK, Nooni IK. The heavy metal contents of some selected medicinal plants sampled from different geographical locations. Pharmacognosy Res. 2013; 5: 103—8.

[36] Karahan F. Evaluation of trace element and heavy metal levels of some ethnobotanically important medicinal plants used as remedies in Southern Turkey in terms of human health risk. Biol Trace Elem Res. 2023; 201: 493-513.

[37] Roy M, McDonald LM. Metal uptake in plants and health risk assessments in metal—contaminated smelter soils. Land Degrad Develop. 2015; 26: 785-92.

[38] Mohamed BA, Ellis N, Kim CS, Bi X. The role of tailored biochar in increasing plant growth, and reducing bioavailability, phytotoxicity, and uptake of heavy metals in contaminated soil. Environ Pollut. 2017; 230: 329—38.

[39] Kulhari A, Sheorayan A, Bajar S, Sarkar S, Chaudhury A, Kalia RK. Investigation of heavy metals in frequently utilized medicinal plants collected from environmentally diverse locations of northwestern India. Springerplus. 2013; 2: 676.

[40] Silva CS, Moutinho C, Ferreira da Vinha A, Matos C. Trace minerals in human health: iron, zinc, copper, manganese and fluorine. Int J Sci Res Methodol. 2019; 13: 57-80.

[41] Giménez VM, Bergam I, Reiter RJ, Manucha W. Metal ion homeostasis with emphasis on zinc and copper: potential crucial link to explain the non—classical antioxidative properties of vitamin D and melatonin. Life Sci. 2021; 281: 119770.

[42] Vogt AC, Arsiwala T, Mohsen M, Vogel M, Manolova V, Bachmann MF. On iron metabolism and its regulation. Int J Mol Sci. 2021; 22: 4591.

[43] Osman D, Cooke A, Young TR, Deery E, Robinson NJ, Warren MJ. The requirement for cobalt in vitamin B₁₂: a paradigm for protein metalation. Biochim Biophys Acta Mol Cell Res. 2021; 1868: 118896.

[44] Rani A, Kumar A, Lal A, Pant M. Cellular mechanisms of cadmium—induced toxicity: a review. Int J Environ Health Res. 2014; 24: 378-99.

[45] Orr SE, Bridges CC. Chronic kidney disease and exposure to nephrotoxic metals. Int J Mol Sci. 2017; 18: 1039.

[46] Kiziler AR, Aydemir B, Onaran I, Alici B, Ozkara H, Gulyasar T, et al. High levels of cadmium and lead in seminal fluid and blood of smoking men are associated with high oxidative stress and damage in infertile subjects. Biol Trace Elem Res. 2007; 120: 82-91.

[47] Odukoya JO, Odukoya JO, Ndinteh DT. Elemental measurements and health risk assessment of subSaharan African medicinal plants used for cardiovascular diseases’ and related risk factors’ treatment. J Trace Elem Med Biol. 2021; 65: 126725.

[48] Liu H, Tang J, Chen T, Zhu P, Sun D, Wang W. Assessment of heavy metals contamination and human health risk assessment of the commonly consumed medicinal herbs in China. Environ Sci Pollut Res. 2023; 30: 7345—57.

[49] Ghasemidehkordi B, Malekirad AA, Nazem H, Fazilati M, Salavati H, Shariatifar N, et al. Concentration of lead and mercury in collected vegetables and herbs from Markazi province, Iran: a noncarcinogenic risk assessment. Food Chem Toxicol. 2018; 113: 204—10. Erratum in: Food Chem Toxicol. 2018;121:717—8.

[50] Burkill HM. The useful plants of west tropical Africa. Econ Bot. 1986; 40: 176.

[51] Soladoye MO, Adetayo MO, Chukwuma EC, Adetunji AN. Ethnobotanical survey of plants used in the treatment of haemorrhoids in South—Western Nigeria. Ann Biol Res. 2010; 1: 1-15.

[52] Aigbe FR, Adeyemi OO. The aqueous root extract of Aristolochia ringens (Vahl) prevents chemically induced inflammation. Planta Med. 2011; 77: PF28.

[53] Van Wyk BE, Wink M, editors. Medicinal plants of the world. Oxfordshire: CABI; 2017.

[54] Neuwinger HD. African ethnobotany: poisons and drugs; chemistry, pharmacology and toxicology. J Nat Prod. 1997; 60: 864—5.

[55] Yakubu MT, Atoyebi AR. Brysocarpus coccineus (Schum & Thonn) root reinstates sexual competence and testicular function in paroxetine—induced sexual dysfunction in male Wistar rats. Andrologia. 2018; 50: e12980.

[56] Sunday EA, Onyeyili PA, Saganuwan SA. Therapeutic effects of Byrsocarpus coccineus root bark extract on bacterially and chemically induced diarrhea in the Wistar albino rat (Rattus norvegicus domestica) . Animal Model Exp Med. 2019; 2: 312—25.

[57] Akindele AJ, Unachukwu EG, Osiagwu DD. 90 Days toxicological assessment of hydroethanolic leaf extract of Ipomoea asarifolia (Desr.) Roem. and Schult. (Convolvulaceae) in rats. J Ethnopharmacol. 2015; 174: 582-94.

[58] Kayode AAA, Kayode OT. Some medicinal values of Telfeiria occidentalis: a review. Am J Biochem. 2011; 1: 30-8.