Eco-friendly synthesis of iron nanoparticles by green tea extract and cytotoxicity effects on tumoral and non-tumoral cell lines

Abstract

Zero-valent iron nanoparticles (ZVI NPs) have been employed in environmental and biomedical applications. These nanoparticles are used in the remediation of soil and water contaminated with heavy metals, and ZVI NPs can be used in the biomedical sector, as drug delivery system due to the large surface area-to-volume ratio. However, their toxicity should be better investigated. In this study, green tea extract was used to synthesize ZVI NPs. The green tea-synthesized nanoparticles (GT-ZVI NPs) were characterized by dynamic light scattering (DLS), X-ray diffraction, Fourier transform infrared spectroscopy and atomic force microscopy. DLS analysis revealed that the nanoparticles have average hydrodynamic size of 104 nm. The results indicated the presence of organic matter derived from green tea extract on the surface of GT-ZVI NPs. The average size of the nanoparticles at solid state was found to be 32 nm. The cytotoxicity of GT-ZVI NPs (1–150 µg/mL) was determined on tumoral (human chondrosarcoma line, SW1353 cells) and non-tumoral (Vero fibroblast cell line) by quantitative cell viability analysis and cell morphological evaluation. GT-ZVI NPs exerted more cytotoxic effect on tumoral line compared to non-tumoral cell line. The viability of SW1353 cells was higher than 75% upon incubation with GT-ZVI NPs, at concentrations in the range of 1–60 µg/mL. At nanoparticle concentrations of 100 and 150 µg/mL, the viability of SW1353 cells decreased 69 and 62%, respectively. These results indicate that these nanoparticles might find important applications in cancer treatment with less toxicity to normal cells.

Keywords

Nanoscale zero-valent iron / Green synthesis / Nanobiotechnology / Cytotoxicity / Cancer cells / Iron nanoparticles

References

[1] Ahamed IN, Anbu S, Vikraman G, Nasreen S, Muthukumari M, Kumar MM. Green synthesis of nano zerovalent iron particles (NZVI) for environmental remediation. Life Sci Arch, 2016, 2: 549-554

[2] Ali I, Peng C, Naz I, Khan ZM, Sultan M, Islam T, Abbasi IA. Phytogenic magnetic nanoparticles for wastewater treatment: a review. RSC Adv, 2017, 7: 40158-40178

[3] Asl BA, Mogharizadeh L, Khomjani N, Rasti B, Pishva SP, Akhtari K, Attar F, Falahati M. Probing the interaction of zero valent iron nanoparticles with blood system by biophysical, docking, cellular, and molecular studies. Int J Biol Macromol, 2018, 109: 639-650

[4] Balachandran C, Sangeetha B, Duraipandiyan V, Raj MK, Ignacimuthu S, Al-Dhabi NA, Balakrishna K, Parthasarathy K, Arulmozhi NM, Valan Arasu M. A flavonoid isolated from Streptomyces sp. (ERINLG-4) induces apoptosis 4 in human lung cancer A549 cells through p53 and cytochrome c release 5 caspase dependant pathway. Chem Biol Interact, 2014, 224: 24-35

[5] Barzan E, Mehrabian S, Irian S. Antimicrobial and genotoxicity effects of zero-valent iron nanoparticles. Jundishapur J Microbiol, 2014, 7: 10054

[6] Cai L, Ye H, Li X, Lin Y, Yu F, Chen J, Li H, Liu X. Chemical constituents of volatile oil from Pyrolae herba and antiproliferative activity against SW1353 human chondrosarcoma cells. Int J Oncol, 2013, 42: 1452-1458

[7] Carbonel GV, Alfieri AF, Alfieri AA, Vidotto MC, Levy CE, Darini ALC, Yanaguita RM. Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens. Cytotoxic activity in S. marcescens. Braz J Med Biol Res, 1997, 30: 1291-1298

[8] Chrysochoou M, Johnston CP, Dahal G. A comparative evaluation of hexavalent chromium treatment in contaminated soil by calcium polysulfide and green-tea nanoscale zero-valent iron. J Hazard Mater, 2012, 33–42: 201-202

[9] Daniel SCGK, Vinothini G, Subramanian N, Nehru K, Sivakumar M. Biosynthesis of Cu, ZVI, and Ag nanoparticles using Dodonaea viscosa extract for antibacterial activity against human pathogens. J Nanopart Res, 2013, 15: 1319

[10] Das RK, Borthakur BB, Bora U. Green synthesis of gold nanoparticles using ethanolic leaf extract of Centella asiatica. Mater Lett, 2010, 64: 1445-1447

[11] Dong H, Li L, Lu Y, Cheng Y, Wang Y, Ning Q, Wang B, Zhang L, Zeng G. Integration of nanoscale zero-valent iron and functional anaerobic bacteria for groundwater remediation: a review. Environ Int, 2019, 124: 265-277

[12] Durán N, Seabra AB. Biogenic synthesized Ag/Au nanoparticles: production, characterization, and applications. Curr Nanosci, 2018, 14: 82-94

[13] Ebrahiminezhad A, Zare-Hoseinabadi A, Sarmah AK, Taghizadeh Z, Ghasemi Y, Berenjian A. Plant-mediated synthesis and applications of iron nanoparticles. Mol Biotechnol, 2018, 60: 154-168

[14] Ezzatahmadi N, Ayoko GA, Millar GJ, Speight R, Yan C, Li J, Li S, Zhu J, Xi Y. Clay-supported nanoscale zero-valent iron composite materials for the remediation of contaminated aqueous solutions: a review. Chem Eng J, 2017, 312: 336-350

[15] Farshchi HK, Azizi M, Jaafari MR, Nemati SH, Fotovat A. Green synthesis of iron nanoparticles by Rosemary extract and cytotoxicity effect evaluation on cancer cell lines. Biocatal Agric Biotechnol, 2018, 16: 54-62

[16] Garcia-Alfonso C, Lopez-Barea J, Sanz P, Repetto G, Repetto M. Changes in antioxidative activities induced by Fe(II) and Fe(III) in cultured Vero cells. Arch Environ Contam Toxicol, 1996, 30: 431-436

[17] Gebauer M, Saas J, Sohler F, Haag J, Söder S, Pieper M, Bartinik E, Beninga J, Zimmer R, Aigner T. Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity to IL-1b1. Osteoarthr Cartil, 2005, 13: 697-708

[18] Gottimukkala KSV, Harika Reddy P, Deeveka Z. Green synthesis of iron nanoparticles using green tea leaves extract. J Nanomed Biother Discov, 2017, 7: 1

[19] Hajsalimi G, Taheri S, Shahi F, Attar F, Ahmadi H, Falahati M. Interaction of iron nanoparticles with nervous system: an in vitro study. J Biomol Struct Dyn, 2017, 36: 928-937

[20] Herlekar M, Barve S, Kumar R. Plant-mediated green synthesis of iron nanoparticles. J Nanopart, 2014

[21] Hoag GE, Collins JB, Holcomb JL, Hoag JR, Nadagouda MN, Varma RS. Degradation of bromothymol blue by ‘greener’ nano-scale zero-valent iron synthesized using tea polyphenols. J Mater Chem, 2009, 19: 8671-8677

[22] Huang L, Weng X, Chen Z, Megharaj M, Naidu R. Green synthesis of iron nanoparticles by various tea extracts: comparative study of the reactivity. Spectrochim Acta A, 2014, 14: 1386-1425

[23] Kaur M, Chopra DS. Green synthesis of iron nanoparticles for biomedical applications. Glob J Nano, 2018

[24] Kumar KM, Mandal BK, Kumar KS, Reddy PS, Sreedhar B. Biobased green method to synthesise palladium and iron nanoparticles using Terminalia chebula aqueous extract. Spectrochim Acta A, 2013, 102: 128-133

[25] Kumar D, Roy R, Parashar A, Raichur AM, Chandrasekaran N, Mukherjee A, Mukherjee A. Toxicity assessment of zero valent iron nanoparticles on Artemia salina. Environ Toxicol, 2017, 32: 1617-1627

[26] Li XQ, Zhang WX. Sequestration of metal cations with zerovalent iron nanoparticles. A study with high resolution X-ray photoelectron spectroscopy (HR-XPS). J Phys Chem, 2007, 111: 6939-6946

[27] Liang W, Li X, Li Y, Li C, Gao B, Huijuan G, Li S, Shen J, Kang J, Ding S, Lin X, Liao L. Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells. Int J Oncol, 2014, 44: 91-98

[28] Lombello CB, Malmonge SM, Wada MLF. PolyHEMA and polyHEMA-poly(MMA-co-AA) as substrates for culturing Vero cells. J Mater Sci Mater Med, 2000, 11: 541-546

[29] Mirzaei S, Hadadi Z, Attar F, Mousavi SE, Zargar SS, Tajik A, Saboury AA, Rezayat SM, Falahati M. ROS-mediated heme degradation and cytotoxicity induced by iron nanoparticles: hemoglobin and lymphocyte cells as targets. J Biomol Struct Dyn, 2017, 36: 4235-4245

[30] Nadagouda MN, Castle AB, Murdock RC, Hussain SM, Varma RS. In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols. Green Chem, 2010, 12: 114-122

[31] Nascimento MHM, Ferreira M, Malmonge SM, Lombello CB. Evaluation of cell interaction with polymeric biomaterials based on hyaluronic acid and chitosan. J Mater Sci Mater Med, 2017, 28: 68

[32] Njagi EC, Huang H, Stafford L, Genuino H, Galindo HM, Collins JB, Hoag GE, Suib SL. Biosynthesis of iron and silver nanoparticles at room temperature using aqueous Sorghum bran extracts. Langmuir, 2010, 27: 264-271

[33] Poguberovic SS, Krcmar DM, Maletic SP, Kónya Z, Pilipovic DDT, Kerkez DV, Roncevic SD. Removal of As(III) and Cr(VI) from aqueous solutions using “green” zero-valent iron nanoparticles produced by oak, mulberry and cherry leaf extracts. Ecol Eng, 2016, 90: 42-49

[34] Rolim WR, Pelegrino MT, Lima BA, Ferraz LS, Costa FN, Bernardes JS, Rodigues T, Brocchi Seabra AB. Green tea extract mediated biogenic synthesis of silver nanoparticles: characterization, cytotoxicity evaluation and antibacterial activity. Appl Surf Sci, 2019, 463: 66-74

[35] Rolim WR, Pieretti JC, Renó DLS, Lima BA, Nascimento MHM, Ambrosio FN, Lombello CB, Brocchi M, Souza ACS, Seabra AB. Antimicrobial activity and cytotoxicity to tumor cells of nitric oxide donor and silver nanoparticles containing PVA/PEG films for topical applications. ACS Appl Mater Interfaces, 2019, 11: 6589-6604

[36] Santos AR Jr, Barbanti SH, Duek EAR, Wada MLF. Analysis of the growth pattern of Vero cells cultured on dense and porous poly (L-Lactic Acid) scaffolds. Mat Res, 2009, 12: 257-263

[37] Seabra AB, Duran N. Nanotoxicology of metal oxide nanoparticles. Metals, 2015, 5: 934-975

[38] Sedaghat Anbouhi T, Mokhtari Esfidvajani E, Nemati F, Haghighat S, Sari S, Attar F, Pakaghideh A, Sohrabi MJ, Mousavi SE, Falahati M. Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles. Int J Nanomed, 2019, 14: 243-256

[39] Shahwan T, Sirriah SA, Nairat M, Boyaci E, Eroglu AE, Scott TB, Hallam KR. Green synthesis of iron nanoparticles and their application as a Fenton-like catalyst for the degradation of aqueous cationic and anionic dyes. Chem Eng J, 2011, 172: 258-266

[40] Siddiqi KS, Rahman AU, Husen A. Biogenic fabrication of iron/iron oxide nanoparticles and their application. Nanoscale Res Lett, 2016, 11: 498

[41] Silva BSO, Seabra AB. Characterization of iron nanoparticles produced with green tea extract: a promising material for nitric oxide delivery. Biointerface Res Appl Chem, 2016, 6: 1280-1287

[42] Singh R, Singh RP, Misra V (2011) Remediation of Cr(VI) contaminated soil by zero-valent iron nanoparticles (nZVI) entrapped in calcium alginate beads. In: ICESD, vol 4. https://doi.org/10.13140/2.1.3293.6969

[43] Srivastava AN, Ahmad R, Khan MA. Evaluation and comparison of the in vitro cytotoxic activity of Withania somnifera methanolic and ethanolic extracts against MDA-MB-231 and Vero cell lines. Sci Pharm, 2016, 84: 41-59

[44] Stefaniuk M, Oleszczuk P, Ok YS. Review on nano zerovalent iron (nZVI): from synthesis to environmental applications. Chem Eng J, 2016, 287: 618-632

[45] Wang T, Jin X, Chen Z, Megharaj M, Naidu R. Green synthesis of Fe nanoparticles using eucalyptus leaf extracts for treatment of eutrophic wastewater. Sci Total Environ, 2014, 466–467: 210-213

[46] Wang T, Lin J, Chen Z, Megharaj M, Naidu R. Green synthesized iron nanoparticles by green tea and eucalyptus leaves extracts used for removal of nitrate in aqueous solution. J Clean Prod, 2014, 83: 413-419

[47] Wang X, Wang P, Ma J, Liu H, Ning P. Synthesis, characterization, and reactivity of cellulose modified nanozero-valent iron for dye discoloration. Appl Surf Sci, 2015, 345: 57-66

[48] Weisburg JH, Weissman DB, Sedaghat T, Babich H. In vitro cytotoxicity of epigallocatechin gallate and tea extracts to cancerous and normal cells from the human oral cavity. Basic Clin Pharmacol Toxicol, 2004, 95: 191-200

[49] Xiao Z, Yuan M, Yang B, Liu Z, Huang J, Sun D. Plant-mediated synthesis of highly active iron nanoparticles for Cr(VI) removal: investigation of the leading biomolecules. Chemosphere, 2016, 150: 357-364

[50] Xie Y, Dong H, Zeng G, Tang L, Jiang Z, Zhanq C, Deng J, Zhang L, Zhang Y. The interactions between nanoscale zero-valent iron and microbes in the subsurface environment: a review. J Hazard Mater, 2017, 321: 390-407

[51] Yirsaw BD, Megharaj M, Chenc Z, Naiduc R. Reduction of hexavalent chromium by green synthesized nano zero valent iron and process optimization using response surface methodology. Environ Technol Innov, 2016, 5: 136-147

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo(2018/08194-2)