Responses of Amaranthus hybridus to effluent from an aluminum plating industry in Lagos, Nigeria

Abstract

The study investigated the responses of Amaranthus hybridus to effluent from an aluminum plating plant in Lagos, Nigeria. Seedlings were raised from seeds and were grouped into three categories, each representing a treatment. The first group which is the control received 250 ml of water via the roots every 3 days, the second category received half-strength effluent via the roots every 3 days, while the third group received full-strength effluent via the roots every 3 days throughout the study period. Physiological and biochemical, as well as metabolic, parameters representative of oxidative damage and antioxidant activity were evaluated after the treatments. The physicochemical analysis of the effluent showed that total suspended solids, Al and Zn levels were higher than the recommended standards. The biomass and the protein content of the control were 18.01 g and 0.835 mg/g dry wt, respectively, as against 12.04 g and 0.368 mg/g dry wt observed in the treated plants. In contrast, effluent treatment caused a significant increase in malondialdehyde level as well as activities of antioxidant enzymes. The present study revealed that the effluent adversely affected growth and development of A. hybridus. Continual discharge of these industrial effluents could have a negative impact on the environment especially to fragile ecosystems.

Keywords

Amaranthus hybridus / Environment / Growth inhibition / Industrial effluent / Toxicity

References

[1] Aebi H. Catalase in vitro. Methods Enzymol, 1984, 105: 121-126

[2] Arnon DI. Copper enzymes in isolated chloroplast, polyphenol-oxidase in Beta vulgaris. Plant Physiol, 1949, 24: 1-15

[3] Ashraf MY, Sarwar G, Ashraf M, Afaf R, Sattar A. Salinity induced changes in α-amylase activity during germination and early cotton seedling growth. Biol Plant, 2002, 45: 589-591

[4] Caplan AB, Dekeyser CR, Van Montagu M. Sangwan RS, Sagwan-Norrel B. Salinity and drought stress in rice. The impact of biotechnology in agriculture, 1990 Amsterdam Kluwer Academic Publishers 391-404

[5] Causton DR. Plant growth analysis: a note on the variability of unit leaf rate (net assimilation rate) within a sample. Ann Bot, 1994, 74: 513-518

[6] Charles J, Sancey B, Morin-Crini N, Badot P, Degiorgi I, Trunfio G, Crini G. Evaluation of the phytotoxicity of polycontaminated industrial effluents using the lettuce plant (Lactuca sativa) as a bioindicator. Ecotoxicol Environ Saf, 2011, 74: 2057-2064

[7] Deepika G, Dhingra HR. Effect of salinity stress on morpho-physiological, biochemical and yield characters of cluster bean [Cyamopsis tetragonoloba (L.) Taub.]. Ind J Plant Physiol, 2014, 19(4): 393-398

[8] Di Salvatore M, Carafa AM, Carratu G. Assessment of heavy metals phytotoxicity using seed germination and root elongation tests: a comparison of two growth substrates. Chemosphere, 2008, 73: 1461-1464

[9] Gardner MJ, Dixon E, Sims I, Whitehouse P. Importance of speciation in aquatic toxicity tests with aluminum. Bull Environ Contam Toxicol, 2002, 68: 195-200

[10] Gomez N, Sierra MV, Cortelezzi A, Capitulo AR. Effects of discharges from the textile industry on the biotic integrity of benthic assemblages. Ecotoxicol Environ Saf, 2008, 69: 472-479

[11] Gopalan HNB. Ecosystem health and human wellbeing: the mission of the international programme plant bioassays. Mutat Res, 1999, 426: 99-102

[12] Guibad G, Gauthier C. Study of aluminum concentration and speciation of surface water in four catchments in the Limousin region (France). J Inorg Biochem, 2003, 97: 16-25

[13] Guo J, Yang Y, Wang G, Yang L, Sun X. Ecophysiological responses of Abies fabri seedlings to drought stress and nitrogen supply. Physiol Plant, 2010, 139: 335-347

[14] Halliwell B, Gutteridge JMC. Free radicals in biology and medicine, 1999 3 New York Oxford Science Publications 936 pp

[15] Han M, Li G, Sang N, Dong Y. Investigating the bio-toxicity of coking wastewater using Zea mays L. assay. Ecotoxicol Environ Saf, 2011, 74(4): 1050-1056

[16] Hossain MA, Rahman GKMM, Rahman MM, Molla AH, Rahman MM, Uddin MK. Impact of industrial effluent on growth and yield of rice (Oryza sativa L.) in silty clay loam soil. J Environ Sci, 2015, 30: 231-240

[17] Kumari M, Tripathi BD. Efficiency of Phragmites australis and Typha latifolia for heavy metal removal from wastewater. Ecotoxicol Environ Saf, 2015, 112: 80-86

[18] Latif MI, Lone MI, Khan KS. Heavy metals contamination of different water sources, soils and vegetables in Rawalpindi area. Soil Environ, 2008, 27(1): 29-35

[19] Lin CF, Hao OJ, Jeng FT. Microtox evaluation of industrial wastewaters. Water Sci Technol, 1994, 30(10): 97-106

[20] Ma TH, Cabrera GL, Owens E. Genotoxic agents detected by plant bioassays. Rev Environ Health, 2005, 20: 1-13

[21] Machlachlan S, Zalik S. Plastid structure chlorophyll concentration and free amino acid composition of a chlorophyll mutant of barley. Can J Bot, 1963, 141: 1053-1062

[22] Nakano H, Muramatsu S, Makino A, Mae T. Relationship between the suppression of photosynthesis and starch accumulation in the pod-removed bean. Aus J Plant Physiol, 2000, 27: 167-173

[23] Nemat Alla MM, Badawi AM, Hassan NM, El-Bastawisy ZM, Badran EG. Effect of metribuzin, butachlor and chlorimuron-ethyl on amino acid and protein formation in wheat and maize seedlings. Pestic Biochem Physiol, 2008, 90: 8-18

[24] Odjegba VJ, Bamgbose NM (2012) Toxicity assessment of treated effluents from a textile industry in Lagos, Nigeria. Afr J Environ Sci Technol 6(11):438–445

[25] Osama A, Floyd MA. Bentazone influence on selected metabolic processes of isolated bean leaf cells. J Plant Growth Regul, 1984, 3: 121-126

[26] Piorreck M, Baasch K, Pohl P. Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue–green algae under different nitrogen regimes. Phytochemistry, 1984, 23: 207-216

[27] Radic S, Stipaniev D, Cvjetko P, Mikelic IL, Rajcic MM, Sirac S, Pevalek-Kozlina B, Pavlica M. Ecotoxicological assessment of industrial effluent using duckweed (Lemna minor L.) as a test organism. Ecotox, 2010, 19: 216-222

[28] Sacan MT, Balcioglu IA (2006) A case study on algal response to raw and treated effluents from an aluminum plating plant and a pharmaceutical plant. Ecotoxicol Environ Saf 64:234–243

[29] Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot, 2001, 52: 2115-2126

[30] Sharma I, Pati PK, Bhardwaj R. Regulation of growth and antioxidant enzyme activities by 28-homobrassinolide in seedlings of Raphanus sativus L. under cadmium stress. Indian J Biochem Biophys, 2010, 47: 172-177

[31] Shi Q, Zhu Z. Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot, 2008, 63: 317-326

[32] Ternes TA. Occurrence of drugs in German sewage treatment plants and rivers. Water Res, 1998, 32: 3245-3260

[33] Turner NC. Techniques and experimental approaches for the measurement of plant water status. Plant Soil, 1981, 58: 339-366

[34] Valerio ME, Garcia JF, Peinado FM. Determination of phytotoxicity of soluble elements in soils, based on a bioassay with lettuce (Lactuca sativa L.). Sci Total Environ, 2007, 378: 63-66

[35] Van Assche F, Clijsters H. Effects of metals on enzyme activity in plants. Plant, Cell Environ, 1990, 13: 195-206

[36] Verma S, Dubey RS. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci, 2003, 164: 645-655

[37] Wang H, Jin JY. Photosynthetic rate, chlorophyll fluorescence parameters, and lipid peroxidation of maize leaves as affected by zinc deficiency. Photosynthetica, 2005, 43: 591-596

[38] Wang YS, Wang J, Yang ZM, Wang QY, Li B, Li SQ, Lu YP, Wang SH, Sun X. Salicylic acid modulates aluminum-induced oxidative stress in roots of Cassia tora. Acta Bot Sin, 2004, 46: 816-828

[39] Yi HL, Meng ZQ. Genotoxicity of hydrated sulfur dioxide on root tips of Allium sativum and Vicia faba. Mutat Res, 2003, 537: 109-114

[40] Yordanova RY, Christov KN, Popova LP. Antioxidative enzymes in barley plants subjected to soil flooding. Environ Exp Bot, 2004, 51(2): 93-101

[41] Zhang F, Wang Z, Dong J. Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguira gymnorrhiza). Chemosphere, 2007, 67: 44-50