ALLО- AND SYMPATRIC SIBLING SPECIES OF SACCHARОMYСES CEREVISIAE: DNA-DNA REASSOCIATION

Yuliya V Mikhailova; Sofia Castello; Elena S Naumova; Gennadiy I Naumov

doi:10.17816/ecogen743-7

Ecological Genetics ›› 2009, Vol. 7 ›› Issue (4) : 3 -7. DOI: 10.17816/ecogen743-7

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ALLО- AND SYMPATRIC SIBLING SPECIES OF SACCHARОMYСES CEREVISIAE: DNA-DNA REASSOCIATION

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Precise DNA-DNA reassociation data were obtained for new biological species S. cariocanus, S. kudriavzevii and S. mikatae, for the first time. The three species showed 25-51 % - of DNA-DNA reassociation with one another and with the known species S. cerevisiae, S. bayanus and S. paradoxus.Only in the combination S. paradoxus × S. cariocanus there was 99 % DNA-DNA homology. Despite high DNA-DNA reassociation value, the two yeasts are genetically isolated: their hybrids are sterile forming non-viable meiotic products (ascospores). Having four reciprocal translocations in its karyotype, S. cariocanus represents species in statu nascendi.

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Yuliya V Mikhailova, Sofia Castello, Elena S Naumova, Gennadiy I Naumov. ALLО- AND SYMPATRIC SIBLING SPECIES OF SACCHARОMYСES CEREVISIAE: DNA-DNA REASSOCIATION. Ecological Genetics, 2009, 7(4): 3-7 DOI:10.17816/ecogen743-7

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References

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[1]	Маниатис Т., Фрич Э., Сэмбрук Дж., 1984. Молекулярное клонирование. Методы генетической инженерии. Москва: Мир, 479 с.

[2]	Наумов Г. И., 1999. Дивергентная популяция дрожжей Saccharomyces paradoxus на Гавайях: вид in statu nascendi//Докл. АН. Т. 364. № 2. С. 281-283.

[3]	Наумов Г. И., 2009а. Гибридологический анализ нового биологического вида Saccharomyces arboricolus Wang et Bai//Докл. АН. Т. 426. № 3. С. 424-426.

[4]	Наумов Г. И., 2009б. Генетическая идентификация дрожжей Saccharomyces kudriavzevii из европейской популяции//Экологическая генетика. Т. 7. № 1. С. 9-11.

[5]	Наумов Г. И., Кондратьева В. И., Наумова Т. И. Гудкова Н. К., 1983. Генетические основы классификации дрожжей Saccharomyces cerevisiae. Изучение выживаемости аскоспор гибридов//Журнал общей биологии. Т. 44. № 5. С. 648-660.

[6]	Britten R. J., Pavich M., Smith J., 1970. A new method of DNA purification//Carnegie Inst. Wash. Year Book. Vol. 68. P. 400-402.

[7]	Fischer G., James S. A., Roberts I. N. Oliver S. G., Louis E. S., 2000. Chromosomal evolution in Saccharomyces//Nature. Vol. 405. P. 451-454.

[8]	Kaneko Y., Banno I., 1991. Reexamination of Saccharomyces bayanus strains by DNA-DNA hybridization and electrophoretic karyotyping//IFO Res. Comm. Vol. 15. P. 30-41.

[9]	Kurtzman C. P., Smiley M. J., Johnson C. J., Wickerham L. J., Fuson G. B., 1980. Two closely related heterothalic species, Pichia amylophyla and Pichia missisipiensis: characterisation by hybridization and deoxyribonucleic acid reassociation//Int. J. Syst. Bacteriol. Vol. 30. P. 208-216.

[10]

Lemos G. A., Valente P., Pimental D., Hagler A. N., Mendonga-Hagler L. C., 1995. Characterization of Saccharomyces paradoxus and a new species of Saccharomyces from Brazilian ecosystem.//Abstracts of VII Int. Symp. Microb. Ecol. (ISME-7). Santos-São Paulo. Brazil. 27 August -01 September. 1995. P. 215.

[11]	Naumov G. I., 1987. Genetic basis for classification and identification of the ascomycetous yeasts//Studies in Mycology. N 30. P. 469-475.

[12]	Naumov G. I., James S. A., Naumova E. S., Louis E. J., Roberts I. N., 2000. Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae//Int. J. Syst. Evol. Microb. Vol. 50. P. 1931 -1942.

[13]	Rodrigues de Sousa M., Madeira-Lopes A., SpencerMartins I., 1995. The significance of active fructose transport and maximum temperature for growth in the taxonomy of Saccharomyces sensu stricto//System. Appl. Microbiol. Vol. 18. P. 44-51.

[14]	Sampaio J. P., Gongalves P., 2008. Natural populations of Saccharomyces kudriavzevii in Portugal are associated with oak bark and are sympatric with S. cerevisiae and S. paradoxus//Appl. Environ. Microbiol. Vol. 74. N 7. P. 2144-2152.

[15]	Seidler R. J., Mandel M., 1971. Quantitative aspects of deoxyribonucleic acid renaturation: base composition, site of chromosome replication, and polynucleotide homologies//J. Bacteriol. Vol. 106. P. 608-614.

[16]	Vaughan Martini A., 1989. Saccharomyces paradoxus comb. nov., a newly separated species of the Saccharomyces sensu stricto complex based upon nDNA/nDNA homologies//System. Appl. Microbiol. Vol. 12. P. 179-182.

[17]	Vaughan Martini A., Kurtzman C. P., 1985. Deoxyribonucleic acid relatedness among species of the genus Saccharomyces sensu stricto//Int. J. Syst. Bacteriol. Vol. 35. P. 508-511.

[18]	Wang S.-A., Bai F.-Y., 2008. Saccharomyces arboricolus sp. nov., a yeast species from tree bark//Int. J. Syst. Evol. Microbiol. Vol. 58. P. 510-514.