Analysis of the role of tonoplast H⁺-ATPase in elongation growth of coleoptile cells of rice seedlings with different growth rates under normoxia and submergence

Abstract

BACKGROUND: Rice coleoptiles were used to investigate the importance of V H⁺-ATPase in vacuolization during elongation growth under normoxic and hypoxic conditions.

AIM of the study was to find out a link between growth intensity, protein amount of subunits B and E and transcription of genes encoding those proteins.

MATERIALS AND METHODS: The investigation was carried out on two rice varieties of domestic selection, fast-growing Kuban 3 and slow-growing Amethyst. Seedlings were grown in etiolated conditions at normoxia and submergence. Western-blot analysis was employed to evaluate amount of subunits B and E in microsomal fraction. qRT-PCR was used to distinguish differences in expression of genes encoding subunits B and E of V H⁺-ATPase.

RESULTS: The growth under aerobic conditions was more consistent with the changes in subunits B and E of V H⁺-ATPase which was determined at the proteomic level, while the hypoxic growth had a stronger correspondence with changes in OsVHAs gene expression. Varietal differences were revealed only when comparing the transcription intensity, which did not affect the growth dynamics of coleoptiles. Obtained data suggested the existence of differences in the regulation of the enzyme at the transcriptional and proteomic levels during coleoptile elongation.

CONCLUSIONS: The importance of the B and E subunits of V-ATPase involvement in vacuolization during the growth process of rice coleoptiles under different oxygen level was demonstrated.

Keywords

hypoxia / rice Oryza sativa L. / vacuolar H+-ATPase

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Funding

Russian Science FoundationРоссийский научный фонд(22-14-00096)

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