Radish (Raphanus sativus L.) as an object of Agrobacterium-mediated transformation

Abstract

Radish (Raphanus sativus L.) is an annual root crop closely related to Arabidopsis thaliana. It can be widely used as a perspective model plant to study the genetic control of storage root development. Thus, obtaining stable genetic transformants of radish to study the function of genes involved in storage root development becomes an important task. In this review, we present the currently available data on Agrobacterium-mediated transformation methods for radish, including in vitro and in planta methods using Agrobacterium tumefaciens, and a method for the production of composite plants with transformed roots or even whole transgenic plants with Agrobacterium rhizogenes. Improvement of existing methods and development of new approaches for radish transformation will increase the productivity of radish varieties, and the wider use of this promising model plant will provide new data on the genetic control of storage root development.

Keywords

radish / Raphanus sativus / transformation / storage root / in vitro transformation / in planta transformation / Agrobacterium-mediated transformation

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