FATE AND ECOLOGICAL INTERACTIONS OF FIREFLY LUC GENE-TAGGED SINORHIZOBIUM MELILOTI 2011-BACTERIA IN SOIL INHABITED BY HIGH LEVELS OF INDIGENOUS ALFALFA NODULATING POPULATIONS
Werner Selbitschka , Mathias Keller , Uve Dresing , Tanja Dammann-Kalinowski , Iren Krahn , Susanne Schneiker-Bekel , D Sch?ffer , W Lotz , Rona Miethling-Graff , Christoph C Tebbe, , Alfred P?hler
Ecological Genetics ›› 2008, Vol. 6 ›› Issue (2) : 61 -71.
FATE AND ECOLOGICAL INTERACTIONS OF FIREFLY LUC GENE-TAGGED SINORHIZOBIUM MELILOTI 2011-BACTERIA IN SOIL INHABITED BY HIGH LEVELS OF INDIGENOUS ALFALFA NODULATING POPULATIONS
A field study was conducted with genetically modified sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA+), both tagged with the firefly luciferase luc gene as an identification marker. The strains' fate was studied over a time period of five years. Both strains were rapidly outcompeted for alfalfa nodulation by an indigenous population. In summary, this study demonstrates the usefulness of tagging bacteria designed for environmental releases by the firefly luciferase gene and the high resilience of soil bacteria to allow the establishment of foreign bacterial populations. This work was supported by grants of the German Federal Ministry of Education and Research (BMBF). We thank Penny Hirsch for valuable discussion concerning the preparation of the peat-based inoculants. The contribution of the Bayer-ische Staatsministerium fur Ernahrung, Landwirtschaft und Forsten for making available the field release site in Stras-smoos is gratefully acknowledged. We thank Rudolf Beck and the team of the Strassmoos experimental plant station for their excellent support during the field study.
Selbitschka W., Keller M., Dresing U., Dammann-Kalinowski T., Krahn I., Schneiker-Bekel S., Sch?ffer D., Lotz W., Miethling-Graff R., Tebbe, C.C., P?hler A.
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