Effect of mutations in the Sym7, Sym19 and Sym34 genes on the interaction of pea (Pisum sativum L.) with the arbuscular mycorrhizal fungus Rhizophagus irregularis

Abstract

BACKGROUND: Within the pea (Pisum sativum L.) species, genotypes with high and low responsiveness to inoculation with arbuscular mycorrhizal fungi can be distinguished.

AIM: The aim of this study was to test the hypothesis that pea responsiveness to arbuscular mycorrhizal fungi inoculation may be inversely correlated with root colonization levels.

MATERIALS AND METHODS: The wild-type line Finale with low responsiveness to arbuscular mycorrhizal fungi inoculation and symbiotic mutants obtained on its basis were used. Plants were grown under controlled climatic conditions with a deficiency of available phosphorus; the fungus Rhizophagus irregularis was used for inoculation. Parameters of plant growth and development of reproductive organs were determined 52 and 71 days after inoculation, which corresponded to the flowering and pod filling stages, respectively.

RESULTS: All mutant lines under conditions without inoculation had generally reduced parameters compared to the original line Finale. Inoculation led to a decrease in many parameters in the line Finale. Mutations in the Sym7 and Sym34 genes, which led to a decrease or delay in the start of mycorrhization, respectively, contributed to the manifestation of a positive plant response to inoculation. The mutant in the Sym19 gene almost completely lacked intrartadical colonization, while inoculation had no effect on the growth and development of above-ground organs.

CONCLUSIONS: The study results support the idea that reducing mycorrhization levels can have a positive effect on pea plants.

Keywords

pea / Pisum sativum L. / arbuscular mycorrhiza / symbiotic mutants / symbiotic effectiveness

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Funding

Российский научный фондRussian Science Foundation (RSF)(22-16-00109)

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