Analysis of arctic char (Salvelinus alpinus) haplotypes in the largest lakes of the North-West Region of Russia for genetic monitoring
Marina N. Kiseleva , Diana K. Mitryushkina , Tatiana A. Filatova , Vasily A. Golotin , Alina A. Zhukova , Konstantin V. Rozhkovan , Anastasia E. Mamaeva , Olga V. Apalikova
Ecological Genetics ›› 2023, Vol. 21 ›› Issue (4) : 357 -368.
Analysis of arctic char (Salvelinus alpinus) haplotypes in the largest lakes of the North-West Region of Russia for genetic monitoring
BACKGROUND: Arctic char is a residential form of the Arctic char Salvelinus alpinus and a commercial target in Ladoga Lake. Caused by fishing the gradual decline in the number of ones in the largest lakes of the North-Western Region of Russia is compensated by measures for its artificial reproduction.
AIM: The aim of this work was to carry out gaplotyping of the arctic char (Salvelinus alpinus) population in the largest lakes of the northwestern region of Russia.
MATERIALS AND METHODS: The gaplotyping by PCR amplification in combination with restriction fragment length polymorphism (PCR-RFLP) of several amplified mtDNA regions, sequencing of the D-loop of mtDNA.
RESULTS: Screening studies of the genetic diversity of arctic char were carried out in three geographical population of the North-Western region of Russia and also the lake form of Arctic char from Sobachye Lake (Putorana Plateau, Western Siberia). The variability of a significant part (more than 35%) of the mitochondrial genome was analyzed including the regions of 16S rRNA/ND1/ND2 (2000 bp), COXI (567 bp), ND5/ND6 (2490 bp) and D-loop (1097 bp). Analysis of the nucleotide sequences of the control region mtDNA revealed 6 different haplotypes that were included in a single Eurasian group of char.
CONCLUSIONS: The panel of PCR-RFLP markers can serve as a tool for monitoring the genetic structure of the arctic char populations of Ladoga Lake under conditions of ones artificial maintenance.
arctic char / Salvelinus alpinus / mitochondrial DNA / PCR / RFLP / sequencing / haplotype
Eco-Vector
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