Modification of agricultural traits in cultivated varieties of barley and wheat

Ekaterina M. Timonova; Antonina A. Kiseleva; Alina A. Berezhnaia; Mikhail A. Nesterov; Irina G. Adonina; Aleksey V. Kochetov; Elena A. Salina

doi:10.17816/ecogen568184

Ecological Genetics ›› : 24 -25. DOI: 10.17816/ecogen568184

Genetically modified organism. The Нistory, Achivements, Social and Environmental Riscs

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Modification of agricultural traits in cultivated varieties of barley and wheat

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Abstract

CRISPR/Cas technology makes it possible to induce mutations at defined positions. In breeding-oriented research, this opens up exciting opportunities for the targeted improvement of many agricultural crops. Wheat and barley are among the most important cereals in the world. However, the transformation poses a particular challenge for cereals and is strongly genotype dependent. This is because agrobacteria, which is mostly used for delivering the CRISPR/Cas system, have a limited compatibility with these non-host plants. Transformation of wheat is additionally difficult due to the large genome size and polyploidy.

Besides obtaining improved genotypes, the object of the current study was to optimize the method of genomic editing based on the CRISPR/Cas system using particle bombardment for non-model varieties of barley and wheat. In barley, we targeted the Nud gene that controls hulled/naked phenotype of the grain. Since the regeneration rate remains an issue for the cultivated cultivars, we used the JD633 vector that carries the GRF4-GIF1 chimera to increase the efficiency of regeneration. We obtained five T₀ plants, carrying mutations. In wheat, targeting Ppd-1 genes that control photoperiod-dependent floral induction results in Cas9-induced mutations in 52 of 210 T₀ plants. The developed collection of wheat plants with different new alleles of Ppd-D1 and Ppd-B1 genes is being studied for the expression under short day conditions and the effect on the vegetation period.

Thus, we have obtained plants of the cultivated varieties of barley and wheat with edited agronomically important genes, using the improved protocols of biolistic transformation.

This work was done within the framework of State Assignment Kurchatov Genomic Center of ICG SB RAS (No. 075-15-2019-1662).

Keywords

CRISPR/Cas / barley / wheat

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Ekaterina M. Timonova, Antonina A. Kiseleva, Alina A. Berezhnaia, Mikhail A. Nesterov, Irina G. Adonina, Aleksey V. Kochetov, Elena A. Salina. Modification of agricultural traits in cultivated varieties of barley and wheat. Ecological Genetics 24-25 DOI:10.17816/ecogen568184