The ability of photochemical decomposition products of the Roundup to induce oxidative stress in bacterial cells

Abstract

BACKGROUND: A common non-selective systemic herbicide Roundup (Glyphosate, active ingredient N-phosphonomethylglycine, N-PMG) is used to control perennial weeds. It is necessary to assess the hazard of the products of photochemical decomposition of N-FMG formed under the influence of solar UV and ozone.

AIM: Using lux-biosensors based on Escherichia coli, studying the ability of N-FMG photochemical degradation products to induce oxidative stress in bacterial cells.

MATERIALS AND METHODS: The work used the active substance of the herbicide Roundup N-phosphonomethylglycine (N-PMG), biosensors E. coli (pSoxS-lux), E. coli (pKatG-lux). UV radiation, Mass spectrometry.

RESULTS: Using biosensors, it was shown that the products of photochemical decomposition of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) cause an increase in the concentration of superoxide anion radical and H₂O₂ in E. coli cells, which induces oxidative stress in the bacterial cell.

CONCLUSIONS: The photochemical decomposition product of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) induces the formation of superoxide anion radical and H₂O₂ in bacterial cells.

Keywords

Glyphosate (Roundup, N-phosphonomethylglycine) / photochemical degradation / Mass spectrometry / E. coli biosensors pSoxS-lux, pKatG-lux / oxidative stress

Funding

Министерство науки и высшего образования Российской ФедерацииMinistry of Science and Higher Education of the Russian Federation(АААА-А19-119071890015-6)

Министерство науки и высшего образования Российской ФедерацииMinistry of Science and Higher Education of the Russian Federation(122022600163-7)

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