Characteristics of morphogenetic responses of Nicotiana langsdorffii on transformation with wild type strains of Agrobacterium in vitro

Abstract

Ability to transformation by wt strains of Agrobacterium tumefaciens (T37, C58, A6) and A. rhizogenes (15834, 8196, A4) was characterized for Nicotiana langsdorffii. It was shown that effectivity of transformation of this species by strains T37, A6,15834, 8196, A4 was lower comparing to the model object N. tabacum. Tumors induced by A. tumefaciens on leaf disks of N. langsdorffii, tend to shoot formation.

Keywords

NICOTIANA LANGSDORFFII / AGROBACTERIUM TUMEFACIENS / Agrobacterium rhizogenes

References

[1] Лакин Г. Ф. Биометрия/Лакин Г. Ф.//Москва: Высшая школа. -1990.

[2] Пирузян Э. С. Основы генетической инженерии растений/Пирузян Э. С.//М: Наука. -1988. -304 с.

[3] Bogani P. A physiological and molecular analysis of the genus Nicotiana/Bogani P., Lio P., Intrieri M.//Molecular Phylogenetics and Evolution. -1997. -Vol. 7 -P. 62-70.

[4] Draper J. Plant genetic transformation and gene expression. A laboratiry manual/Draper J., Scott R.//Blackwell Scientific Publications. -1988. -408 p.

[5] Goodspeed T. H., Ed. "The genus Nicotiana" Waltham, 1954.

[6] Ichikawa T. Tobacco genetic tumors./Ichikawa T., Syono K.//Plant Cell Physiol. -1991 Vol. 32 (8) -P. 1123-1128.

[7] Intrieri M. C. The horizontal transfer of Agrobacterium rhizogenes genes and evolution of the genus Nicotiana/Intrieri M. C., Buiatti. M.//Molecular Phylogenetics and evolution. 2001. -Vol. 20, N 1. -P. 100-110.

[8] Jin S. Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281./Jin S., Komari Т., Gordon M. P., Nester E. W//J. Bacteriol. -1987. -Vol. 169, N 10. -P 4417-4425.

[9] Knauf V. C. Comparison of Ti plasmids from three different biotypes of Agrobacterium tumefaciens isolated from grapevines/Knauf V C., Panagopoulos C. G., Nester E. W.//J. Bacteriol. -1983. -Vol. 153, N 3. -P. 1535-42.

[10] Limited-host-range plasmid of Agrobacterium tumefaciens: molecular and genetic analyses of transferred DNA./Yanofsky M., Montoya A., Knauf V. [et al.]//J. Bacteriol. -1985. -Vol. 163, N 1. -P. 341-348.

[11] Murashige T. A revised medium for rapid growth and bioassay with tobacco tissue culture/Murashige T., Skoog F.//Physiol. Plant. -1962. -Vol. 15.

[12] Otten L. Evolution of agrobacteria and their Ti plasmids -a review./Otten L., Canaday J., Gerard J. C. [et al.]//Mol. Plant-Microbe Interact. -1992 -Vol. 5, N 4. -P. 279-287.

[13] Sequence homologous to Agrobacterium rhizogenes T-DNA in the genomes of uninfected plants./F. F. White, D. J. Garfinkel, G. A. Huffman [et al.]//Nature, 1983. -Vol. 301. -P. 348-350.

[14] White F. F. Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome./Ghidossi G., Gordon M. P., Nester E. W.//Proc Natl Acad Sci U S A. -1982. -Vol. 79 (10). -P. 3193-3197.

RIGHTS & PERMISSIONS

Matveeva T.V., Pigichka T.Y., Lutova L.A.