Assessment of variability of egg production traits based on analysis of SNP markers and search for traces of selection in the genome of Russian white chickens

Abstract

Objective. To assess the variability of egg production traits for nine SNPs, to search for traces of selection in the genome of Russian white chickens based on ROH patterns.

Methods. The material for the study was DNA isolated from the blood of Russian white chickens (n = 141). Nine SNPs associated with egg production at p < 5.16 · 10^–5 according to GWAS data were selected for analysis. The frequencies of alleles and genotypes, the relationship between genotypes and characteristics of egg production were calculated, and ROH patterns were identified.

Results. Significant differences between genotypes were found in terms of age of laying the first egg (p < 0.005) and egg weight (p < 0.05). The genomic regions surrounding the target SNPs were analyzed according to the distribution of homozygous regions in them.

Conclusions. The substitutions rs317565390 and rs16625488 located in the 4.8–10.2 Mb region on chromosome 8 showed polymorphism, despite the fact that homozygous loci in this region of the genome are found in 58% of animals. For most SNPs, the prevalence of the frequency of one of the alleles was observed. As a cluster of increased selection pressure, a chick genome region in the 4.8–10.2 Mb region on chromosome 8 was identified.

Keywords

Gallus gallus / age of first egg laying / egg weight / local breeds / ROH patterns / polymorphic loci / SNP

Funding

Российский фонд фундаментальных исследованийRussian Foundation for Basic Research(18-016-00114)

RIGHTS & PERMISSIONS

Mitrofanova O., Dementieva N., Fedorova E.S., Pozovnikova M., Tyshchenko V., Scherbakov Y., Plemyashov K.