The dissociation of Bacillus thuringiensis as manifestation of «stationary-phase mutagenesis»
Valentina I Chemerilova , Olga A Sekerina , Elena V Kravets , Larisa V Malanushenko
Ecological Genetics ›› 2006, Vol. 4 ›› Issue (1) : 28 -35.
The dissociation of Bacillus thuringiensis as manifestation of «stationary-phase mutagenesis»
Particularities of the morphological R-variants appearance in periodic culture of two strains of Bacillus thuringiensis were investigated. It is shown that both in optimum and in stressful conditions (рН 9,4) the R-variant frequency was increased in stationary phase only. Results from Luria and Delbrück's fluctuation test established that the frequency of R-variants is independent of the number generations passed and the number of colonies under investigation. Majority of R-variants was asporogenous and acrystalligenous, but didn't differ from original S-variants in set of others diagnostic characteristics that are significant for type. Mating between S- and R-variants has shown that the presence of R-variants in culture provides increasing to efficiency of recombination between of the subspecies.
Chemerilova V.I., Sekerina O.A., Kravets E.V., Malanushenko L.V.
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