Analysis of stable chromosome aberrations, revealed with FISH-method in veterans of special risk troops

Abstract

The frequency of stable chromosomal aberrations, detected by FISH, was found to be significantly higher in group of persons undergone low-dose irradiation (Semipalatinsk, Novaya Zemlya, South Ural) as compared to age-matched control group. The age accumulation of a stable chromosome aberrations in an age range 45-76 years was more expressed in exposed group than in control one

References

[1] Воробцова И.Е., Такер Дж. Д., Тимофеева Н.М. и др. Влияние возраста и облучения на частоту транслокаций и дицентриков, определяемых методом FISH, в лимфоцитах человека.//Радиационная Биология. Радиоэкология. -2000. -Т. 40, № 2. -С. 142-148.

[2] Пилинская М.А. Дыбский С.С. Частота хромосомных обменов в критических группах жертв Чернобыльской аварии по данным традиционного цитогенетического анализа и метода FISH//Межд. журн. радиац. медиц. -2000. -№ 1. -С. 83-95

[3] Святова Г.С., АбильдиноваГ.Ж., Березина Г.М. Результаты цитогенетического исследования популяций различного радиационного риска Семипалатинского региона//Генетика. -2002. -Т. 38, №3. -С. 376-382

[4] Снигирева Г.П., Богомазова А.Н., Хазинс ЕД. Организация цитогенетического обследования лиц, пострадавших в результате радиационных аварий.//Методические указания № 99/134. М. -1999.-33 с.

[5] Шкварова Т.Г. Изучение нестабильных и стабильных аберраций хромосом у работников ядерно-химических предприятий и лиц с острой лучевой болезнью в отдаленный пострадиационный период: Автореф. дис.... канд. биол. наук. -Обнинск, 2002. -22 с.

[6] Akleyev А. V., Lyubchansky E.R. Environmental and medical effects of nuclear weapon production in the Southern Urals//Sci. of the Tot. Envir. -1994. -Vol. 142. -P. 1-8.

[7] Awa A. Analysis of chromosome aberrations in atomic bomb survivors for dose assessment: studies at the radiation effects research foundation from 1968 to 1993//Stem Cells. -1997. -Vol. 2. -P. 163-173.

[8] Bauchinger M. Braselmann H., Savage J.R.K. et al. Collaborative exercise on the use of FISH chromosome painting for retrospective biodosimetry of Mayak nuclear-industrial personnel//Int. J. Radiat. Biol. -2001. -Vol. 77. -P. 259-267.

[9] Hagmar L., BroggerA., Hansteenl.L. Cancer risk in humans predicted by increased levels of chromosomal aberrations in lymphocytes: Nordic study group on the health risk of chromosome damage//Cancer Research. -1994. -Vol. 54. -P. 2919-1922. Jones I.M.,

[10] Galick H., Kato P. etal. Three somatic genetic biomarkers and covariates in radiation-exposed Russian cleanup workers of the Chernobyl nuclear reactor 6-13 years after exposure//Radiat. Res. -2002. -Vol. 158. -P. 424-442.

[11] Koshurnikova N.A., Buldakov L.A., Bysogolov G.D. et al. Mortality from malignancies of the hematopoetic and lymphatic tissues among personnel of the first nuclear plant in the URSS//Sci. of the Total Envir. -1994. -Vol. 142. -P. 19-23.

[12] Lindholm C, Tekkel M., Weidenbaum T. et al. Persistence of translocations after accidental exposure to ionizing radiation//Int. J. Radiat. Biol. -1998. -Vol. 74. -P. 565-571.

[13] Lucas J.N., Awa A., Straume Т. et al. Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation//Int. J. Radiat. Biol. -1992. -Vol. 62. -P. 53-63.

[14] Natarajan А.Т., Santos S.J., Darroudi F et al. '"Cesium-induced chromosome aberrations analyzed by fluorescence in situ hybridization: eight years follow up of the Goiania radiation accident victims//Mutat. Res. -1998. -Vol. 400. -P. 299-312.

[15] Okladnikova N., Tokarskaja Z. and Musatkova O. Cytogenetic effect of239 Pu incorporation and external gamma radiation (clinical investigation)//Medical Radiol. -1994. -Vol. 5. -P. 48-52.

[16] Pinkel D., Straume T. and Gray J.W. Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization//Proceed, of the Nat. Acad, of Sci. USA. -1986. -Vol. 83. -P. 2934-2938.

[17] Ramsey M.J., Moore 11 D.H., Briner J.F. et al. The effects of age and lifestyle factors on the accumulation of cytogenetic damage as measured by chromosome painting//Mutat. Res. -1995. -Vol. 338. -P. 293-298.

[18] Salassidis K, Georgiadou-Schumacher V., Braselmann H et al. Chromosome painting in highly irradiated Chernobyl victims: a follow-up study to evaluate the stability of symmetrical translocations and influence of clonal aberrations for retrospective dose estimation//Int. J. Radiat. Biol. -1995. -Vol. 68. -P. 257-262.

[19] Salassidis K., Braselmann Н., Okladnikova N.D. et al. Analysis of symmetrical translocations for retrospective biodosimetry in radiation workers of the Mayak nuclear-industrial complex (Southern Urals) using FISH-chromosome painting//Int. J. Radiat. Biol. -1998. -Vol. 74. -P. 431-439.

[20] Salomaa S., Sevan'kaev A. V., Zhloba A.A. et al. Unstable and stable chromosome aberrations in lymphocytes of people exposed to Chernobyl fallout in Bryansk Russia//Int. J. Radiat. Biol. -1997. -Vol. 71. -P. 51-59.

[21] Salomaa S., Lindholm C., Tankimanova MX. et al. Stable chromosome aberrations in the lymphocytes of a population living in the Semipalatinsk nuclear test site//Radiat. Res. -2002. -Vol. 158. -№ 5. -P. 591-596.

[22] Simon S.L., Baverstock K.F., Lindholm C. A summary of evidence on radiation exposures received near to the Semipalatisk nuclear weapons test site in Kazakhstan.//Health Phys. -2003. -Vol. 86, N 6. -P. 718-725.

[23] Snigiryova G, Braselmann H., Salassidis K. et al. Retrospective biodosimetry of Chernobyl clean-up workers using chromosome painting and conventional chromosome analysis//Int. J. Radiat. Biol. -1997-Vol. 71-P. 119-127.

[24] Sorokine-Durm I., Whitehouse C., Edwards E. The variability of translocation yields amongst control populations//Radiat. Prot. Dosim. -2000. -Vol. 88. -P. 93-99.

[25] Stephan G, Pressl S., Koshpessova G. Analysis of FISH-painted chromosome in individuals living near the Semipalatinsk nuclear test site.//Radiat. Res. -2001. -Vol. 155. -P. 794-798.

[26] Tucker J.D., Lee D.A., Ramsey M.J. etal. On the frequency of chromosome exchanges in a control population measured by chromosome painting//Mutat. Res. -Vol. 113. -P. 193-202.

[27] Vorobtsova I., Semenov A., Timqfeyeva N. et al. An investigation of the age-dependency of chromosome abnormalities in human populations exposed to low-dose ionizing radiation//Mech. of Aging and Develop. -2001, -Vol. 122. -P. 1373-1382.

RIGHTS & PERMISSIONS

Vorobcova I.E., Lubimova N.E., Perova A.A., Semenov A.V.