Somaclonal variability of conifers in culture in vitro
Elena N. Gulyaeva , Roman V. Ignatenko , Natalia A. Galibina
Ecological Genetics ›› 2020, Vol. 18 ›› Issue (3) : 301 -315.
Somaclonal variability of conifers in culture in vitro
The use of somatic embryogenesis is one of the promising methods of conifer propagation on an industrial scale. However, this technology has a number of problems, which include the appearance of somaclonal variation in cell and tissue culture. The review considers the causes and methods for detecting somaclonal variability of conifer in culture in vitro. It is shown that it is necessary to use a complex of molecular, cytogenetic, morphological, physiological methods for the analysis of somaclonal changes in embryogenic plant cultures.
somaclonal variability / propagation of conifers / somatic embryogenesis / cryopreservation
Gulyaeva E.N., Ignatenko R.V., Galibina N.A.
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