The analysis of the polyamine oxidase genes in the methylotrophic yeast Komagataella phaffii

Abstract

Polyamines are present in all living cells and regulate a wide range of biological processes. In Saccharomyces cerevisiae the polyamine oxidase Fms1p converts spermine to spermidine and 3-aminopropionaldehyde, which is necessary for the synthesis of pantothenic acid and hypusination. This paper shows that S. cerevisiae FMS1 gene orthologs are present in all major representatives of the Saccharomycotina subdivision, but their copy numbers are different. In the Komagataella phaffii (Pichia pastoris) yeast, two polyamine oxidase genes (KpFMS1 and KpFMS2) were identified, and the regulation of their promoters activity was studied.

Keywords

polyamine oxidases / methylotrophic yeast / Komagataella phaffii / Pichia pastoris

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Funding

RFBRРФФИ()

RIGHTS & PERMISSIONS

Ivanova A.V., Sidorin A.V., Sambuk E.V., Rumyantsev A.M.