Influence of γ-irradiation on the expression of encoding ABA metabolism enzymes in barley embryos
Sofia V. Bitarishvili , Vladimir S. Bondarenko , Stanislav A. Geras’kin
Ecological Genetics ›› 2018, Vol. 16 ›› Issue (4) : 85 -89.
Influence of γ-irradiation on the expression of encoding ABA metabolism enzymes in barley embryos
Background. Small doses of radiation stimulate the growth and development of plants including seed germination. ABA plays a key role not only in seed dormancy and germination but also in the regulation of adaptive reactions of plants.
The aim of our work was to study the effect of γ-irradiation of barley seeds in a small doses on the expression of genes encoding ABA biosynthesis enzyme 9-cis-epoxycarotenoid dioxygenase (HvNCED1) and catabolism enzyme ABA 8’-hydroxylase (HvABA8’OH-1).
Materials and Methods. The barley seeds were irradiated at dose range of 4–50 Gy at a dose rate of 60 Gy/h, the radiation source was 60Co. The study was carried out in the embryos within the first 30 hours after germination. Relative gene expression was investigated using real-time PCR (RT-PCR).
Results. It was shown that γ-irradiation of barley seeds changes the expression of ABA biosynthesis and catabolism genes in all experimental groups.
Conclusion. This alterations can lead to a decrease the ABA content under irradiation with stimulating doses and increase the biosynthesis of phytohormone under irradiation with inhibitory dose.
barley / γ-irradiation / ABA / HvNCED1 / HvABA8’OH / real-time PCR / gene expression
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Bitarishvili S.V., Bondarenko V.S., Geras’kin S.A.
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