Specificity of the symbiotic interaction of bacteria of the genus Rhizobium leguminosarum bv. viciae with plants of the tribe Vicieae

Abstract

The estimation of nodulation competitiveness of industrial strains against the native nodule bacteria and also the analyses of distribution of strain’s genotypes which formed nodules on roots of 4 plant species was the purpose of this work. The objects of the research were rhizobium strains which formed nodules on roots of plant (the nodule-forming units – NFU), obtained in field experiment with application of preseeding processing of seeds of pea (Pisum satіvum L.), fava beans (Vicia faba L.), lathyrus (Lathyrus sativus L.) and lentil (Lens culinaris L.). The mixture of the collection strains allocated from nodules of peas and beans, and having various combinations of chromosomal and symbiotic genotypes was used for inoculation of seeds. Identification of NFU was carried out with the use of the analysis of the emitted nodule total DNA on several chromosomal markers: the rpoB gene and the hin-region, and the plasmid marker – nodD gene. It is established that only about 50% of nodules were formed by the strains used at inoculation of seeds. Besides, the combinations of chromosomal and symbiotic genotypes specific for a rhizobium – symbionts of concrete host plants have been established: IA genotype with sym-2 for P. sativum; IB genotype with sym-4 for V. faba. The results of this study create prerequisites for selection of couples: macro- and microsymbiont for the purpose of increasing efficiency of plant-microbial systems, in which the nature of symbiotic interaction defines efficiency of partners.

Keywords

Rhizobium leguminosarum bv. viciae / Pisum / Vicia / Lathyrus / Lens / rpoB / hin-region / nodD / specificity

Funding

Ministry of Education and Science of the Russian FederationМинистерство образования и науки Российской Федерации()

RIGHTS & PERMISSIONS

Khapchaeva S.A., Didovich S.V., Topunov A.F., Mulyukin A.L., Zotov V.S.