Analysis of introgressive lines of inter-species pea hybrids by band composition of seed proteins

Abstract

Background. The reproductive incompatibility of cultivated (Pisum sativum) and wild (P. fulvum) pea species determines the difficulties of obtaining hybrids as well as the transfer of valuable wild parent alleles into interspecific hybrids and their use in the breeding process. The aim of the research was a comparative study of protein spectra of pea interspecific hybrids BC₂F₅ P. sativum × P. fulvum obtained by the authors and their parents.

Materials and methods. The band composition of seed proteins in the interspecific hybrids of peas BC₂F₅, variety Stabil (P. sativum) × accession from VIR collection I-609881 (P. fulvum) has been studied. Effectiveness of parent gene transfer determining each polymorphic position of electrophoretic spectrum were evaluated.

Results. The ratio of the actual frequencies of the bands of the cultivated and wild parents in the introgression lines corresponded to the expected level in 73% positions of the electrophoretic spectrum. The introgression rate of individual seed protein bands from wild parent into interspecific pea hybrids in the absence of selection significantly exceeded the expected level, which may indicate the adaptive value of alleles encoding unique seed protein isoforms.

Conclusion. The possibility of introgressive transfer of wild-type alleles to the cultivated genotypes of pea, as well as the presence of identified cultivated isoforms of storage proteins in all studied lines of BC₂F₅ interspecific hybrids in 88.2% of the polymorphic positions of the electrophoretic spectrum, indicates the possibility of using the wild species P. fulvum in pea breeding.

Keywords

peas / Pisum sativum / P. fulvum / interspecific hybrid / SDS-PAGE / introgression / protein / protein band / isophorm

Funding

ФГБНУ «Федеральный научный центр зернобобовых и крупяных культур»Federal Scientific Center of Legumes and Groat Crops,(Array)

Федеральный исследовательский центр «Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)Federal Research Center “All-Russian Institute of Plant Genetic Resources named after N.I. Vavilova (VIR)(Array)

RIGHTS & PERMISSIONS

Bobkov S.V., Bychkov I.A., Selikhova T.N., Semenova E.V., Vishnyakova M.A.