Low level of genetic differentiation among populations of the rare species Allium regelianum A.K. Becker ex Iljin from the Volgograd region detected by ISSR-analysis
Aya A Trifonova , Elena Z Kochieva , Alexander M Kudryavtsev
Ecological Genetics ›› 2017, Vol. 15 ›› Issue (1) : 30 -37.
Low level of genetic differentiation among populations of the rare species Allium regelianum A.K. Becker ex Iljin from the Volgograd region detected by ISSR-analysis
Background. Knowledge of genetic diversity within and among populations of rare and endangered plants species is practically important for conservation management. Molecular markers are useful tools for analysis of genetic diversity. In this study ISSR-analysis of rare endemic species Allium regelianum which grows in the Volgograd region was performed for the first time.
Materials and Methods. A total of 93 samples from the 11 populations were collected and used in analysis. Six primers used in ISSR-analysis. Data analysis was performed using the GenAlEx 6.41, POPGEN 1.32, PAST 3.11 and STRUCTURE 2.3.1 programs.
Results. A total of 109 ISSR-fragments were scored of which 87 (79.8%) were polymorphic. Comparatively high level of intrapopulation diversity was estimated for the population of the area near Krasnoyarskii khutor, Serafimovichskii administrative region, and for the population of Khrenovatyi Liman, Nikolaevskii administrative region of Volgograd Oblast. Genetic similarity index among populations ranged from 0.88 to 0.96. Genetic differentiation among populations of A. regelianum, GST was 0.284, only. Analysis of molecular variance showed that genetic heterogeneity of A. regelianum 83% was attributed to differences within populations and 17% occurred among populations. Principal coordinate analysis and analysis of populations structure (with the used of STRUCTURE program) found no clear differentiation among populations.
Conclusion. The estimation of intra- and interpopulation diversity of A. regelianum was performed. ISSRs detected high levels of genetic similarity within the populations of A. regelianum and low level of genetic differentiation among populations.
Allium regelianum / Allium regelianum / rare and endangered species / genetic diversity / ISSR-analysis
Trifonova A.A., Kochieva E.Z., Kudryavtsev A.M.
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