The assessment of micronucleus frequency in lymphocytes in the cohort of coal-miners characterized by different polymorphisms of double strand break reparation genes

Abstract

Background: Coal-miners are exposed to a lot of number of harmful factors (chemical agents, ionizing radiation, heavy metals, coal dust etc.). Material and methods: Venous blood samples extracted from 129 coal-miners. Assessment of cytogenetic damage was performed using the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes. PCR and gel electrophoresis were used to determine polymorphisms in the genes Lig4 (rs1805388) and XRCC4 (rs6869366). Results: We found a significant increase in the frequency of binucleated lymphocytes with micronuclei (MN) and protrusions in carriers of the Ile/ Ile genotype of the Lig4 gene Thr9Ile polymorphism in comparison to Thr/Thr and Thr/Ile genotypes. Conclusions: Thr9Ile polymorphism within Lig4 gene can be used as potential molecular genetic markers of increased individual susceptibility to the complex of harmful factors in coal-mining conditions.

Keywords

cytogenetics / micronucleus assay / coal / genotyping / DNA-reparation

References

[1] Закс Л. (1976) Статистическое оценивание. М.: Статистика.

[2] Ингель Ф. И. (2006) Перспективы использования микроядерного теста на лимфоцитах крови человека, культивируемых в условиях цитокинетического блока. Часть I. Пролиферация клеток. Экологическая генетика. Т. 3 (3): С. 7-19.

[3] Fenech M. (1993) The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutation Research. V. 285: P. 35-44.

[4] Fenech M. (2000) The in vitro micronucleus technique. Mutation Research. V. 455: P. 81-95.

[5] Fenech M., Kirsch-Volders M., Natarajan A. T. et al. (2011) Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis. V. 26: P. 125-132.

[6] Donbak L., Rencuzogullari E., Yavuz A., Topaktas M. (2005) The genotoxic risk of underground coal miners from Turkey. Mutation Research. V. 588: P. 82-87.

[7] Kvitko K., Bandinelli E., Henriques J. et al. (2012) Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining. Genetics and Molecular Biology. V. 35: P. 1060-1068.

[8] León-Mejía G., Espitia-Pérez L., Hoyos-Giraldo L. S. et al. (2011) Assessment of DNA damage in coal open-cast mining workers using the cytokinesis-blocked micronucleus test and the comet assay. Science of the Total Environment. V. 409: P. 686-691.

[9] León-Mejía G., Quintana M., Debastiani R. et al. (2014) Genetic damage in coal miners evaluated by buccal micronucleus cytome assay. Ecotoxicology and Environmental Safety. V. 107: P. 133-139.

[10] Rohr P., Kvitko K., da Silva F. R. et al. (2013) Genetic and oxidative damage of peripheral blood lymphocytes in workers with occupational exposure to coal. Mutation Research. V. 758: P. 23-28.

RIGHTS & PERMISSIONS

Sinitsky M.Y., Volobaev V.P., Asanov M.A.