The assessment of micronucleus frequency in lymphocytes in the cohort of coal-miners characterized by different polymorphisms of double strand break reparation genes
Maxim Yur'yevich Sinitsky , Valentin P. Volobaev , Maxim Aydarovich Asanov
Ecological Genetics ›› 2015, Vol. 13 ›› Issue (4) : 30 -33.
The assessment of micronucleus frequency in lymphocytes in the cohort of coal-miners characterized by different polymorphisms of double strand break reparation genes
Background: Coal-miners are exposed to a lot of number of harmful factors (chemical agents, ionizing radiation, heavy metals, coal dust etc.). Material and methods: Venous blood samples extracted from 129 coal-miners. Assessment of cytogenetic damage was performed using the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes. PCR and gel electrophoresis were used to determine polymorphisms in the genes Lig4 (rs1805388) and XRCC4 (rs6869366). Results: We found a significant increase in the frequency of binucleated lymphocytes with micronuclei (MN) and protrusions in carriers of the Ile/ Ile genotype of the Lig4 gene Thr9Ile polymorphism in comparison to Thr/Thr and Thr/Ile genotypes. Conclusions: Thr9Ile polymorphism within Lig4 gene can be used as potential molecular genetic markers of increased individual susceptibility to the complex of harmful factors in coal-mining conditions.
cytogenetics / micronucleus assay / coal / genotyping / DNA-reparation
Sinitsky M.Y., Volobaev V.P., Asanov M.A.
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