Obtaining of interspecific hybrids for pea introgressive breeding

Abstract

Background. Overcoming of reproductive isolation, identification and transfer of agronomic value genes from wild relatives into cultivated pea genomes is an important task for pea introgressive breeding. Materials and methods. Reciprocal hybridization of cultivated pea with wide set of P. fulvum accessions was conducted. Identification of hybrids was carried out with use of biochemical and morphological markers. Identification of unique protein was conducted with use of electrophoretic spectra of mature seeds. Results. Pea interspecific hybrids were obtained in two reciprocal directions of crosses. Cross efficiency in Р. sativum × P. fulvum and P. fulvum × Р. sativum combinations was 36 % and 7 %, respectively. All tested seeds in crosses Р. sativum × P. fulvum were hybrids. Crosses in direction P. fulvum × Р. sativum led to formation of puny seeds restricted in embryo growth. Protein markers of one seed derived in cross P. fulvum × Р. sativum proved its hybrid nature. Morphological markers demonstrated that plant derived from another cross was also a hybrid. Culture of immature embryos was developed for recovering plants in interspecific crosses. Morphogenic calli and regenerated plants were obtained in culture of immature embryos P. fulvum (И592589) × Р. sativum (Aest). Identification of unique protein 7 of P. fulvum was conducted. Inheritance of that protein was proved as monogenic dominant. Conclusion. Efficiency of hybridization in combination P. fulvum × Р. sativum was significantly less in compare to reciprocal one. All products of that cross combination were tested as hybrids. Unique protein 7 of P. fulvum was revealed as a result of mature seed electrophoretic spectra analysis. Inheritance of that protein was determined as monogenic dominant.

Keywords

Pisum fulvum / pea / wild species / Pisum fulvum / interspecific hybridization / SDS-PAGE electrophoresis / embryo / storage protein / introgression / lipoxygenase

RIGHTS & PERMISSIONS

Bobkov S.V., Selikhova T.N.