Influence of phytoecdysteroids and plants steroidal glycosides on the lifespan and stress resistance of drosophila melanogaster
Mikhail Vyacheslavovich Shaposhnikov , Lyubov' Alekseevna Shilova , Ekaterina Nikolaevna Plyusnina , Svetlana Olegovna Volodina , Vladimir Vital'yevich Volodin , Aleksey Aleksandrovich Moskalev
Ecological Genetics ›› 2014, Vol. 12 ›› Issue (4) : 3 -14.
Influence of phytoecdysteroids and plants steroidal glycosides on the lifespan and stress resistance of drosophila melanogaster
Background. Elucidation of the molecular mechanisms of effects of the active substances of plant adaptogens is a topical area of researches. Materials and methods. We studied the effect of herbal substances containing phytoecdysteroids (20-hydroxyecdysone and inokosterone) of Serratula coronata L. or steroidal glycosides (dioscin and protodioscine) of Trigonella foenum-graecum L. on the expression level of stress response genes (genes of heat shock proteins, DNA repair, antioxidant defense and apoptosis), stressresistanse (paraquat, starvation, hyperthermia) and lifespan of Drosophila melanogaster. Results. The studied herbal substances upregulated genes of antioxidant defense mechanisms (Sod1), but downregulated the DNA repair (XPF and Rad51) and apoptosis (Hid) genes. At the same time herbal substances induced weak adaptogenic and antiaging effects. Conclusion. Our results demonstrate that the herbal substances containing phytoecdysteroids and steroidal glycosides change the expression level of stress-response genes and activate mechanisms of hormesis.
Drosophila / phytoecdysteroids / plants steroidal glycosides / adaptogens / lifespan / stress resistance / hormesis / Drosophila
Shaposhnikov M.V., Shilova L.A., Plyusnina E.N., Volodina S.O., Volodin V.V., Moskalev A.A.
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