Population genetic structure of HLA-G gene in North-west Region of Russian Federation

Abstract

Background. HLA-G is a non-classical MHC class Ib molecule predominantly expressed in cytotrophoblasts and under pathological conditions also in chronically inflamed and in malignant tissues. Polymorphic sites present in coding and non-coding regions of the HLA-G gene may potentially affect all of these biological features. Materials and methods: Using the PCR method we have studied polymorphism of HLA G gene (alleles G*0101-G0107, -725C/G , 3741del/ins14.) in 118 people aged from 20 till 40 years of the North-West Region of Russia (St. Petersburg). Results: To determine the common alleles of the HLA-G gene the system of allele-specific PCR primers was elaborated. We have determined HLA-G allele distribution in North-west Region of Russian Federation. The minor allele frequencies varied in wide ranges - from 0 % to 39 %. The genetic distances among the Spanish, Chinese, Japanese, Danish, Indian, African Shona, Brazilian, Polish, German and North-west Region of Russian Federation were calculated. Conclusion: The evaluation of alleles rates in North-west of Russian Federation and in populations of other countries revealed the similarity in alleles G*0101-G0107 and polymorphism 3741del/ins14 distribution among the Danish, German and North-west Region populations.

Keywords

HLA-G / population analysis / HLA-G / alleles / multiplex PCR / genetic distances / northwest region of the Russian Federation

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RIGHTS & PERMISSIONS

Alenichev A.P., Nasykhova Y.A., Ivashchenko T.E., Baranov V.S.