Search for SNP markers of stress tolerance in the TaDREB1 and TaWRKY19 genes of bread wheat in the Cis-Ural steppe zone

Abstract

BACKGROUND: Under the conditions of the Cis-Ural steppe zone, abiotic stress factors such as winter conditions and drought have a negative impact on the yield of bread wheat (Triticum aestivum L.). However, the efficiency of breeding to these stress factors is limited by wide variations depending on the weather conditions in different years. To collect stress-resistant forms in the selected material, genomic selection can be used, however, SNP markers of drought tolerance and winter hardiness of bread wheat, unique to this region, are unknown.

AIM: The purpose of this study is to identify SNP markers in the genes of the key transcription factors of bread wheat TaDREB1 and TaWRKY19, which are involved in the regulation of plant resistance to abiotic stresses.

MATERIALS AND METHODS: 16 varieties and lines of bread wheat were analyzed, contrasting in terms of winter hardiness and drought tolerance. DNA from wheat leaves was isolated by the standard CTAB method. Primers were selected, fragments of open reading frames of the studied genes were amplified, and nucleotide sequences were determined by automatic capillary sequencing.

RESULTS: Among the analyzed bread wheat varieties and lines, 12 SNPs in the TaDREB1 gene and 5 SNPs in TaWRKY19 were found.

CONCLUSIONS: The results of statistical analysis showed that substitution 866 T/A in the TaDREB1 gene is associated with winter hardiness, while substitution 587 A/G in the TaWRKY19 gene is associated with winter hardiness and drought resistance.

Keywords

Triticum aestivum L. / SNP / drought / winter hardiness / stress tolerance / transcription factors

References

[1] Gahlaut V, Jaiswal V, Kumar A, Kumar Gupta P. Transcription factors involved in drought tolerance and their possible role in developing drought tolerant cultivars with emphasis on wheat (Triticum aestivum L.). Theor Appl Genet. 2016;129:2019–2042. DOI: 10.1007/s00122-016-2794-z

[2] Gahlaut V., Jaiswal V., Kumar A., Kumar Gupta P. Transcription factors involved in drought tolerance and their possible role in developing drought tolerant cultivars with emphasis on wheat (Triticum aestivum L.) // Theor Appl Genet. 2016. Vol. 129. P. 2019–2042. DOI: 10.1007/s00122-016-2794-z

[3] Würschum T, Longin CFH, Hahn V, et al. Copy number variations of CBF genes at the Fr-A2 locus are essential components of winter hardiness in wheat. Plant J. 2017;89(4):764–773. DOI: 10.1111/tpj.13424

[4] Würschum T., Longin C.F.H., Hahn V., et al. Copy number variations of CBF genes at the Fr-A2 locus are essential components of winter hardiness in wheat // Plant J. 2017. Vol. 89, No. 4. Р. 764–773. DOI: 10.1111/tpj.13424

[5] Kolchanov NA, Kochetov AV, Salina EA, et al. Sostoyanie i perspektivy ispolzovaniya marker-orientirovannoi i genomnoi selektsii rastenii. Vestnik Rossijskoj Akademii Nauk. 2017;87(4):348–354. (In Russ.) DOI: 10.7868/S0869587317040107

[6] Колчанов Н.А., Кочетов А.В., Салина Е.А., и др. Состояние и перспективы использования маркер-ориентированной и геномной селекции растений // Вестник Российской академии наук. 2017. Т. 87, № 4. С. 348–354. DOI: 10.7868/S0869587317040107

[7] Baillo EH, Kimotho RN, Zhang Z, Xu P. Transcription factors associated with abiotic and biotic stress tolerance and their potential for crops improvement. Genes. 2019;10(10):771. DOI: 10.3390/genes10100771

[8] Baillo E.H., Kimotho R.N., Zhang Z., Xu P. Transcription factors associated with abiotic and biotic stress tolerance and their potential for crops improvement // Genes. 2019. Vol. 10, No. 10. ID 771. DOI: 10.3390/genes10100771

[9] Pellegrineschi A, Reynolds M, Pacheco M, et al. Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome. 2004;47(3):493–500. DOI: 10.1139/g03-140

[10] Pellegrineschi A., Reynolds M., Pacheco M., et al. Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions // Genome. 2004. Vol. 47, No. 3. Р. 493–500. DOI: 10.1139/g03-140

[11] Niu C-F, Wei W, Zhou Q-Y, et al. Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants. Plant, Cell Environ. 2012;35(6):1156–1170. DOI: 10.1111/j.1365-3040.2012.02480.x

[12] Niu C.-F., Wei W., Zhou Q.-Y., et al. Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants // Plant, Cell Environ. 2012. Vol. 35, No. 6. Р. 1156–1170. DOI: 10.1111/j.1365-3040.2012.02480.x

[13] Zaikina EA, Musin KhG, Kuluev AR, et al. Izmenenie aktivnosti genov transkriptsionnykh faktorov TANAC69, TADREB1, TABZIP60 u rastenii myagkoi pshenitsy pri vodnom defitsite i gipotermii. Fiziologiya rastenij. 2022;69(3):327–336. (In Russ.) DOI: 10.31857/S0015330322030186

[14] Заикина Е.А., Мусин Х.Г., Кулуев А.Р., и др. Изменение активности генов транскрипционных факторов TANAC69, TADREB1, TABZIP60 у растений мягкой пшеницы при водном дефиците и гипотермии // Физиология растений. 2022. Т. 69, № 3. С. 327–336. DOI: 10.31857/S0015330322030186

[15] Hu X, Ren J, Ren X, et al. Association of agronomic traits with SNP markers in durum wheat (Triticum turgidum L. durum (Desf.)). PloS One. 2015;10(6). DOI: 10.1371/journal.pone.0130854

[16] Hu X., Ren J., Ren X., et al. Association of agronomic traits with SNP markers in durum wheat (Triticum turgidum L. durum (Desf.)) // PloS One. 2015. Vol. 10, No. 6. DOI: 10.1371/journal.pone.0130854

[17] Metodika gosudarstvennogo sortoispytaniya selskokhozyaistvennykh kultur. Vyp. 2. Moscow, 1989. 196 p. (In Russ.)

[18] Методика государственного сортоиспытания сельскохозяйственных культур. Вып. 2. Москва, 1989. 196 c.

[19] Doyle JJ, Doyle JL, Rapid А. DNA Isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1987;19(1):1–11.

[20] Doyle J.J., Doyle J.L., Rapid А. DNA Isolation procedure for small quantities of fresh leaf tissue // Phytochem Bull. 1987. Vol. 19, No. 1. Р. 1–11.

[21] Sakuma Y, Maruyama K, Qin F, et al. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. PNAS USA. 2006;103(49):18822–18827. DOI: 10.1073/pnas.0605639103

[22] Sakuma Y., Maruyama K., Qin F., et al. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression // PNAS USA. 2006. Vol. 103, No. 49. Р. 18822–18827. DOI: 10.1073/pnas.0605639103

[23] Seki M, Narusaka M, Abe H, et al. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell. 2001;13(1):61–72. DOI: 10.1105/tpc.13.1.61

[24] Seki M., Narusaka M., Abe H., et al. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray // Plant Cell. 2001. Vol. 13, No. 1. Р. 61–72. DOI: 10.1105/tpc.13.1.61

[25] Dubouzet JG, Sakuma Y, Ito Y, et al. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J. 2003;33(4):751–763. DOI: 10.1046/j.1365-313x.2003.01661.x

[26] Dubouzet J.G., Sakuma Y., Ito Y., et al. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression // Plant J. 2003. Vol. 33, No. 4. Р. 751–763. DOI: 10.1046/j.1365-313x.2003.01661.x

[27] Zhou Q-Y, Tian A-G, Zou H-F, et al. Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants. Plant Biotechnol J. 2008;6(5):486–503. DOI: 10.1111/j.1467-7652.2008.00336.x

[28] Zhou Q.-Y., Tian A.-G., Zou H.-F., et al. Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants // Plant Biotechnol J. 2008. Vol. 6, No. 5. Р. 486–503. DOI: 10.1111/j.1467-7652.2008.00336.x

[29] Wang C-T, Ru J-N, Liu Y-W, et al. Maize WRKY transcription factor ZmWRKY106 confers drought and heat tolerance in transgenic plants. Int J Mol Sci. 2018;19(10):3046. DOI: 10.3390/ijms19103046

[30] Wang C.-T., Ru J.-N., Liu Y.-W., et al. Maize WRKY transcription factor ZmWRKY106 confers drought and heat tolerance in transgenic plants // Int J Mol Sci. 2018. Vol. 19, No. 10. ID3046. DOI: 10.3390/ijms19103046

[31] Jaiswal V, Gahlaut V, Mathur S, et al. Identification of novel SNP in promoter sequence of TaGW2-6A associated with grain weight and other agronomic traits in wheat (Triticum aestivum L.). PLoS One. 2015;10(6). DOI: 10.1371/journal.pone.0129400

[32] Jaiswal V., Gahlaut V., Mathur S., et al. Identification of novel SNP in promoter sequence of TaGW2-6A associated with grain weight and other agronomic traits in wheat (Triticum aestivum L.) // PLoS One. 2015. Vol. 10, No. 6. DOI: 10.1371/journal.pone.0129400

Funding

Государственные заданияState assignments(№122030200143-8, FMRS-2022-0130)

Грант Минобрнауки РФGrant of the Ministry of Education and Science of the Russian Federation(№ 075-15-2021-1066 от 28 сентября 2021 г.)

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