Using the SSAP analysis for the primary localization of the cdt (cadmium tolerance) mutation in pea linkage group VI
Viktor E Tsyganov , Olga A Kulaeva , Maggie Knox , Aleksey U Borisov , Igor A Tikhonovich , Tomas N Ellis
Ecological Genetics ›› 2012, Vol. 10 ›› Issue (1) : 46 -50.
Using the SSAP analysis for the primary localization of the cdt (cadmium tolerance) mutation in pea linkage group VI
To localize the cdt mutation leading to an increased cadmium tolerance in the pea mutant and an increased cadmium accumulation in the biomass, F2 and F3 progenies from crosses between the mutant SGECdt line and the JI 281 line were analyzed. The joint inheritance of 89 SSAP (sequence specific amplified polymorphism) markers, by which the analyzed lines differed, and the mutant trait of cadmium tolerance was performed. The linkage between the trait of cadmium tolerance and 4 SSAP markers: Tps1/146+, Tps1/167+,Tps1/44+ and Tps1/58+, localized in VI pea linkage group, was shown. Thus, prospects of using SSAP analysis for primary localization of a mutation in the linkage group were demonstrated.
Tsyganov V.E., Kulaeva O.A., Knox M., Borisov A.U., Tikhonovich I.A., Ellis T.N.
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