Fine mapping of a cdt locus mutation that leads to increased cadmium tolerance
Olga A Kulaeva , Viktor E Tsyganov
Ecological Genetics ›› 2012, Vol. 10 ›› Issue (1) : 38 -45.
Fine mapping of a cdt locus mutation that leads to increased cadmium tolerance
A pea mutant SGECdt (cdt), which has an increased cadmium tolerance and an increased cadmium accumulation, as compared to the initial line, was recently obtained. Earlier, a SSAP (sequence specific amplified polymorphism) analysis revealed localization of the cdt locus in VI linkage group. For fine mapping of the cdt locus a set of PCR based markers was developed. PCR markers were based on known sequences of pea genes, which were determined using analysis of genome microsynteny between pea and model legume Medicago truncatula. The close linkage of the cdt locus and markers based on the Pentatricopeptide repeat and Exosome complex exonuclease RRP 45 genes was revealed. Thus, prerequisites for cdt positional cloning were developed.
Kulaeva O.A., Tsyganov V.E.
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