Novel series os pea symbiotic mutants induced in the SGE line

Abstract

 Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of М₂ progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fix^– phenotype), 13 mutantsunable to form nodules (Nod^– phenotype), and 2 mutants forming a few nodules (Nod^+/– phenotype). For 1 Nod^– and 5 Fix^– mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fix^–mutant SGEFix^––9 an additional mutation leading to Nod^+/– phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFix<span style="font-size:11px">^- - </span>5 line is caused by a mutation in the sym33 gene, of theSGEFix^––6 linein the sym40 gene, of the SGEFix^––7 line in the sym27 gene, and of the SGEFix^––8 linein the sym25 gene.

Keywords

PISUM SATIVUM L

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RIGHTS & PERMISSIONS

Tsyganov V.E., Voroshilova V.A., Rozov S.M., Borisov A.U., Tikhonovich I.A.