Field-deployable, ultrasensitive and visual detection systems targeting severe fever with thrombocytopenia syndrome virus (Dabie bandavirus) based on CRISPR/Cas12a and DNAzyme

Junhu Wang; Ming Yue; Yingqing Mao; Qiong Chen; Yixin Ge; Haiming Yi; Hongming Wang; Sunjie Yang; Jin Zhu; Yuexi Li; Yong Qi

doi:10.1002/ctm2.70393

Clinical and Translational Medicine ›› 2025, Vol. 15 ›› Issue (7) : e70393 DOI: 10.1002/ctm2.70393

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Field-deployable, ultrasensitive and visual detection systems targeting severe fever with thrombocytopenia syndrome virus (Dabie bandavirus) based on CRISPR/Cas12a and DNAzyme

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Junhu Wang, Ming Yue, Yingqing Mao, Qiong Chen, Yixin Ge, Haiming Yi, Hongming Wang, Sunjie Yang, Jin Zhu, Yuexi Li, Yong Qi. Field-deployable, ultrasensitive and visual detection systems targeting severe fever with thrombocytopenia syndrome virus (Dabie bandavirus) based on CRISPR/Cas12a and DNAzyme. Clinical and Translational Medicine, 2025, 15(7): e70393 DOI:10.1002/ctm2.70393

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References

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[1]	Liu S, Chai C, Wang C, et al. Systematic review of severe fever with thrombocytopenia syndrome: virology, epidemiology, and clinical characteristics. Rev Med Virol. 2014; 24(2): 90-102.

[2]	Yun SM, Park SJ, Kim YI, et al. Genetic and pathogenic diversity of severe fever with thrombocytopenia syndrome virus (SFTSV) in South Korea. JCI Insight. 2020; 5(2): e129531.

[3]

World Health Organization, 2018 Annual review of diseases prioritized under the research and development blueprint. World Health Organization; 2018. https://www.who.int/news-room/events/detail/2018/02/06/default-calendar/2018-annual-review-of-diseases-prioritized-under-the-research-anddevelopment-blueprint

[4]	Zhang Y, Bai X, Li J, et al. A CRISPR-based nucleic acid detection method for severe fever with thrombocytopenia syndrome virus. Virus Res. 2022; 311: 198691.

[5]	Li J, Cheng H, Wang X, et al. Rapid, portable Epstein‒Barr virus DNA detection using enzymatic recombinase amplification combined with the CRISPR-Cas12a system. Clin Transl Med. 2024; 14(9): e70028.

[6]	Li X, Zhu S, Zhang X, et al. Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes. Front Bioeng Biotechnol. 2023; 11: 1215466.

[7]	Varghese J, De Silva I, Millar DS. Latest advances in arbovirus diagnostics. Microorganisms. 2023; 11(5): 1159.

[8]	Pandey R, Lu Y, McConnell EM, et al. Electrochemical DNAzyme-based biosensors for disease diagnosis. Biosens Bioelectron. 2023; 224: 114983.

[9]	Park BJ, Yoo JR, Heo ST, Kim M, Lee KH, Song YJ. A CRISPR-Cas12a-based diagnostic method for multiple genotypes of severe fever with thrombocytopenia syndrome virus. PLoS Negl Trop Dis. 2022; 16(8): e0010666.

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2025 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.