The discovery of Cas9's trans-cleavage activity: Unlocking new molecular diagnostic tools

Ying Chen; Jiyun Chen; Liang Liu

doi:10.1002/ctm2.70384

Clinical and Translational Medicine ›› 2025, Vol. 15 ›› Issue (6) : e70384 DOI: 10.1002/ctm2.70384

INVITED LETTER

The discovery of Cas9's trans-cleavage activity: Unlocking new molecular diagnostic tools

Author information +

History +

PDF

Keywords

Cas9 / Trans-cleavage

Cite this article

Download citation ▾

Ying Chen, Jiyun Chen, Liang Liu. The discovery of Cas9's trans-cleavage activity: Unlocking new molecular diagnostic tools. Clinical and Translational Medicine, 2025, 15(6): e70384 DOI:10.1002/ctm2.70384

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Kaminski MM, Abudayyeh OO, Gootenberg JS, et al. CRISPR-based diagnostics. Nat Biomed Eng. 2021; 5(7): 643-656.

[2]	Hu C, Ni D, Nam KH, et al. Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Mol Cell. 2022; 82(15): 2754-2768.e2755.

[3]	Swarts DC, Jinek M. Mechanistic insights into the cis- and trans-acting DNase activities of Cas12a. Mol Cell. 2019; 73(3): 589-600.e584.

[4]	Abudayyeh OO, Gootenberg JS, Konermann S, et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016; 353(6299): aaf5573.

[5]	Gootenberg JS, Abudayyeh OO, Lee JW, et al. Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017; 356(6336): 438-442.

[6]	Chen JS, Ma E, Harrington LB, et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science. 2018; 360(6387): 436-439.

[7]	Chen J, Chen Y, Huang L, et al. Trans-nuclease activity of Cas9 activated by DNA or RNA target binding. Nat Biotechnol. 2025; 43(4): 558-568.

[8]	Broughton JP, Deng X, Yu G, et al. CRISPR-Cas12-based detection of SARS-CoV-2. Nat Biotechnol. 2020; 38(7): 870-874.

[9]	Jinek M, Chylinski K, Fonfara I, et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337(6096): 816-821.

RIGHTS & PERMISSIONS

2025 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.