In vitro reconstitution of SPO11-mediated DNA cleavage reveals mechanistic and clinical insights into meiotic DNA double-strand break formation

Zetao Hu; Xinzhe Tang; Ming-Han Tong; Ying Huang

doi:10.1002/ctm2.70383

Clinical and Translational Medicine ›› 2025, Vol. 15 ›› Issue (6) : e70383 DOI: 10.1002/ctm2.70383

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In vitro reconstitution of SPO11-mediated DNA cleavage reveals mechanistic and clinical insights into meiotic DNA double-strand break formation

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germ cell / infertility / meiosis / SPO11

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Zetao Hu, Xinzhe Tang, Ming-Han Tong, Ying Huang. In vitro reconstitution of SPO11-mediated DNA cleavage reveals mechanistic and clinical insights into meiotic DNA double-strand break formation. Clinical and Translational Medicine, 2025, 15(6): e70383 DOI:10.1002/ctm2.70383

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References

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[6]	Tang X, Hu Z, Ding J, et al. In vitro reconstitution of meiotic DNA double-strand-break formation. Nature. 2025; 639(8055): 800-807.

[7]	Zheng Z, Zheng L, Arter M, et al. Reconstitution of SPO11-dependent double-strand break formation. Nature. 2025; 639(8055): 784-791.

[8]	Oger C, Bouuaert CC. SPO11 dimers are sufficient to catalyse DNA double-strand breaks in vitro. Nature. 2025; 639(8055): 792-799.

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2025 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.