Unravelling the prognostic and operative role of intratumoural microbiota in non-small cell lung cancer: Insights from 16S rRNA and RNA sequencing

Abstract

	•Four phyla, five classes, nine orders, 17 families and 36 genera have been found associated with NSCLC prognosis.
	•We identified a protective microbial cluster associated with delayed recurrence and a harmful microbial cluster related to shorter survival and earlier recurrence.
	•We identified Peptococcus as an independent, detrimental prognostic factor for NSCLC, potentially impacting prognosis via TNF signalling.

Keywords

microbiota / non-small cell lung cancer (NSCLC) / Peptococcus / prognosis / protective and harmful microbial clusters

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2025 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.