Molecular characterization of human HSPCs with different cell fates in vivo using single-cell transcriptome analysis and lentiviral barcoding technology

Abstract

	•SCALeBa and its algorithm are developed to study the molecular mechanism underlying human HSPCs identity and function.
	•The human HSPCs expressing MYL6B, MYO19, ATP2A2, MDN1, ING3, and PHF20 may have the capability for high stemness.
	•The human HSPCs expressing COA3, RIF1, RAB14, and GOLGA4 may have the capability for pluripotent-lineage differentiation.
	•The human HSPCs expressing MRPL23 and RBM4 genes may have the capability to differentiate into myeloid and lymphoid lineage respectively in vivo.
	•The legitimacy of the identified genes with SCALeBa was validated using biological experiments and a public human HSPCs dataset.
	•SCALeBa improves the accuracy of differentiation trajectories in monocle2-based pseudo-time analysis.

Keywords

barcoding technology / hematopoietic stem and progenitor cells / lineage tracing / scRNA-seq

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RIGHTS & PERMISSIONS

2024 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.