Rapid, portable Epstein–Barr virus DNA detection using enzymatic recombinase amplification combined with the CRISPR–Cas12a system

Jia Li , Hao Cheng , Xiaojun Wang , Ning Chen , Liujie Chen , Lili Duan , Fenghua Tan , Kai Li , Duanfang Liao , Zheng Hu

Clinical and Translational Medicine ›› 2024, Vol. 14 ›› Issue (9) : e70028

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Clinical and Translational Medicine ›› 2024, Vol. 14 ›› Issue (9) : e70028 DOI: 10.1002/ctm2.70028
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Rapid, portable Epstein–Barr virus DNA detection using enzymatic recombinase amplification combined with the CRISPR–Cas12a system

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Jia Li, Hao Cheng, Xiaojun Wang, Ning Chen, Liujie Chen, Lili Duan, Fenghua Tan, Kai Li, Duanfang Liao, Zheng Hu. Rapid, portable Epstein–Barr virus DNA detection using enzymatic recombinase amplification combined with the CRISPR–Cas12a system. Clinical and Translational Medicine, 2024, 14(9): e70028 DOI:10.1002/ctm2.70028

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References

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Tsao SW, Tsang CM, Lo KW. Epstein-Barr virus infection and nasopharyngeal carcinoma. Philos Trans R Soc Lond B Biol Sci. 2017; 372(1732): 20160270.

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Patchsung M, Jantarug K, Pattama A, et al. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020; 4(12): 1140-1149.

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Chen JS, Ma E, Harrington LB, et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science. 2018; 360(6387): 436-439.

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Ding X, Yin K, Li Z, Liu C. All-in-one dual CRISPR-Cas12a (AIOD-CRISPR) assay: a case for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 and HIV virus. bioRxiv: the preprint server for biology. 2020. doi:10.1101/2020.03.19.998724

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Li J, Wang Y, Hu J, Bao Z, Wang M. An isothermal enzymatic recombinase amplification (ERA) assay for rapid and accurate detection of Enterocytozoon hepatopenaei infection in shrimp. J Invertebr Pathol. 2023; 197: 107895.

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Deng Z, Hu H, Tang D, et al. Ultrasensitive, specific, and rapid detection of Mycoplasma pneumoniae using the ERA/CRISPR-Cas12a dual system. Front Microbiol. 2022; 13: 811768.

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2024 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

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